VEGF蛋白及mRNA在乳腺癌组织中的表达及意义

Expression and significance of VEGF protein and its mRNA in the tissues of breast cance

目的:探讨血管内皮生长因子(VEGF)蛋白及mRNA在乳腺癌发生、发展过程中的临床意义。方法:运用免疫组化EnvisionTM和半定量RT-PCR的方法检测56例乳腺癌和30例乳腺纤维腺瘤组织中VEGF蛋白及mRNA的表达情况,并分析其与临床病理参数的关系。结果:VEGF蛋白在乳腺癌组织中阳性表达率为75.0%(42/56),在乳腺纤维腺瘤组织中为23.3%(7/30);mRNA VEGF在乳腺癌和乳腺纤维腺瘤组织中表达水平分别为(0.8207±0.1042)、(0.3275±0.0384)灰度比值,差异有统计学意义(P<0.05);VEGF蛋白及mRNA的表达与组织学分级和淋巴结转移呈正相关(P<0.05)。结论:VEGF蛋白及mRNA参与乳腺癌的发生、发展过程,可作为评估乳腺癌浸润与淋巴结转移的重要生物学指标。

Objective To study expression of vascular endothelial growth factor(VEGF) protein and its mRNA in the breast cancer and evaluate its significance to clinicopathology of this tumor invasion and metastasis.Methods The Immunohistochemical staining method of EnvisinTM and reverse transcription polymerase chain reaction(RT-PCR) were used to evaluate the expression of VEGF and its mRNA in 56 cases of breast cancer and 30 cases of benign breast diseases and to analyze its correlations to clinicopathologic features.Results Comparing with the the expression that was 23.3 %(P <0.05) in benign breast diseases,The positive incidence of VEGF protein expression in 56 cases of breast cancer was 75.0%(42/56),The levels of VEGF mRNA were significantly higher in the patients with breast cancer was(0.8207±0.1042),comparing with those in benign breast diseases [(0.3275±0.0384),P <0.05].The expression of VEGF was positively correlated to axillary lymph node metastasis and pathological stage(P <0.05).Conclusion The VEGF protein and mRNA were associated in human breast cancers and could serve as an important index in the aspect of invasiveness and metastasis in breast cancer.