8-溴-7-甲氧基白杨素增强三氧化二砷对白血病U937细胞毒性

bromo-7-methoxychrysin enhances Arsenic Trioxide induced cytotoxicity in human leukemia U937 cell lines

目的:研究8-溴-7-甲氧基白杨素增强三氧化二砷(Arsenic Trioxide,ATO)对急性单核细胞白血病U937细胞毒性作用。方法:MTT比色法检测细胞毒性,流式细胞仪(FCM)测定细胞凋亡率,Western blot分析Bax和Bcl-XL蛋白表达。结果:2.0?M BrMC和ATO联合处理U937细胞的毒性作用显著高于单用相同浓度BrMC或ATO(P<0.05)。在BrMC和ATO联合作用下,U937细胞凋亡率、Bax蛋白表达显著增高,Bcl-XL蛋白表达显著降低。结论:BrMC具有增强ATO对白血病U937细胞毒性作用,其作用机制涉及上调Bax/Bcl-XL比值促进细胞凋亡。

Objective To investigate whether 8-bromo-7-methoxychrysin(BrMC) sensitizes Arsenic Trioxide(ATO) mediated cytotoxicity in human acute monocytic leukemia U937 cell line.Methods The cytotoxicity was examined by MTT assay.FCM was used to determine apoptosis rate.Western blot was used to analyze expression of Bax and Bcl-XL protein.Results The cytotoxicity of U937 cells by the cotreatment with 2.0 μM of BrMC and ATO was significantly higher than that of BrMC or ATO alone.Combination of BrMC and ATO could increase apoptosis rate and the ratio of Bax/Bcl-XL in U937 cells.Conclusion BrMC significantly sensitizes ATO_induced cytotoxicity in leukemia U937 cell lines,which is associated with potentiation of apoptosis through increase of the ratio of Bax/Bcl-XL.