HPLC法测定三七止血胶囊中三七总皂苷含量研究

Determination of Saponins Content in panax notoginseng capsule With HPLC

目的:完善三七止血胶囊的质量标准,控制药品质量。方法:采用高效液相色谱法测定三七止血胶囊中三七皂苷R1﹑人参皂苷Rg1﹑人参皂苷Rb1的含量。色谱柱:色谱柱:C18Alltima 250×4.6 mm,5μL;流动相:以流动相A:乙腈,流动相B:水,采用梯度洗脱方式测定,检测波长:203nm;流速:1.0mL/min。结果:测定10批样品,三七皂苷的平均含量为3.04 mg/粒,三七皂苷R1线性范围为0.1008～0.9072μg,相关线性系数:R=0.9999,人参皂苷Rg1线性范围为0.4160～3.7440μg,相关线性系数:R=0.9999,人参皂苷Rb1线性范围为0.4136～3.7224μg。相关线性系数:R=0.9999,平均回收率:94.41%,RSD:0.74%(n=5)。结论:建立的方法简便易行,重现性好,可有效的控制该制剂的质量。

Objective To perfect the quality panax notoginseng and to control its quality.Methods Determination of the contents of ginsenoside Rg_1,Rb_1 and the notoginsenoside R_1 in the total notoginsenosides of the Panax notogineseng by HPLC,Column:separated on C18 column(250 × 4.6mm Alltima,5 μL) using a gradient mobile phase consisting of acetonitrile-water buffer.Results Determination of 10 batches of samples,the average recovery was 3.04 mg / tablets,ginsenoside Rg1 showed a good linear correlation in the range of 0.4160 to 3.7440μg(R=0.9999),the average recovery rate was 94.41%,and RSD 0.74%(n=5) Conclusion The method is simple,feasible and reproducible this study provides a effective method for controling of the quality of preparation.