脂质过氧化反应混合物诱发人羊膜FL系细胞程序外DNA合成

The reaction mixture of lipid peroxidation inducing unscheduled DNA synthesis in human amnion FL cells

大鼠肝微粒体依赖NADP的脂质过氧化反应混合物(LPRM)可诱发人羊膜FL系细胞程序外DNA合成(UDS)。当一定浓度的LPRM(0.7～2.4mg微粒体蛋白/ml)加入^(14)C—TdR预标记的FL细胞培基时,引起羟基尿素(HU)抗性的程序外~3H—TdR掺入,与对照组相比差异显著(P<0.05),并存在剂量—效应关系。由此可见,脂质过氧化反应混合物中含有诱变物,推测其在癌肿发病中可能有直接作用。

The reaction mixture of NADPH-dependent lipid peroxidation of rat liver micro-somes induces"unscheduled DNA synthesis（UDS）" in human amnion FL cells. The re-sults obtained were as follows: （1）Lipid peroxidation of microsomes occurred extensi-vely in the incubation with NADPH and Fe2+. （2） In the FL cells, UDS level incres-ed markedly when the cells were incubated in the reaction microsomal lipid perox-idation.The induced UDS were dependent on the extent of the lipid peroxidation.According to the results, it is concluded that some DNA-damaging and mutagenic su-bstances are indeed produced in the degradation process of peroxidized polyunsaturat-ed fatty acids in liver microsomal lipids.