皮状丝孢酵母Trichosporon cutaneum产油脂发酵培养基优化

Optimization of Fermentation Medium for Lipid Production from Trichosporon cutaneum

对皮状丝孢酵母发酵产油脂培养基中的碳氮源种类及添加量、KH_2PO_4添加量、接种量等影响因素进行优化。试验表明,葡萄糖为最佳碳源,蛋白胨为最佳氮源,在葡萄糖40 g/L,蛋白胨1.33 g/L,KH_2PO_4质量浓度1.0 g/L,最佳接种量15%时,油脂得率可达到细胞干质量的62%,比优化前提高了50%。

The effective factors of carbon and nitrogen source, carbon and nitrogen amount, KH：PO4 amount, different inoculation volume on lipid production from Trichosporon cutaneum are optimized. The results show that the glucose is the best carbon source, peptone is the best nitrogen source. The lipid yield can be up to 62% under the conditions of glucose 40 g/L, peptone 1.33 g/L, KH2PO4 1.0 g/L, the inoculation volume 15% with increasing 50% than before.