重楼皂苷Ⅰ对人肝癌细胞MHCC97-H增殖、周期、凋亡的影响

Effects of Polyphyllin Ⅰ and Sorafenib on the Proliferation and Cell Cycle and Apoptosis of MHCC97-H

目的观察重楼皂苷Ⅰ对人肝癌细胞MHCC97-H增殖、周期及凋亡的影响。方法 1.将对数生长期的人肝癌细胞MHCC97-H,加入不同浓度的重楼皂苷Ⅰ和索拉菲尼,24h后CCK8法检测药物对细胞增殖的影响。2.根据CCK8实验结果,选取相应浓度的重楼皂苷Ⅰ和索拉菲尼,作用于细胞24h,观察细胞周期及凋亡情况。结果重楼皂苷Ⅰ及索拉非尼作用于人肝癌细胞MHCC97-H的IC50的浓度分别是5.7μg/m L、10μmol/m L。重楼皂苷Ⅰ及索拉菲尼均能抑制S期,将细胞阻滞于G0/G1期,能明显促进细胞凋亡,与空白对照组比较具有显著差异(P<0.01)。结论重楼皂苷Ⅰ对人肝癌细胞MHCC97-H具有明显的抑制增殖及促进凋亡的作用。

Objective To observe effects of Polyphyllin I and Sorafenib on the proliferation and cell cycle and apoptosis of MHCC97-H. Methods MHCC97H cells of logarithmic growth phase was detected for cell proliferation by adding different concentrations of Polyphyllin I and sorafenib for 24 hours. 2. According to the results of CCK8, the concentration of IC50 of Polyphyllin I and Sorafenib on MHCC97-H cells was selected and interfering cells for 24 hours to observe the cell cycle and apoptosis. Results IC50 ofPolyphyllin I and sorafenib on MHCC97H cells was 5.7μg/ml、 10μmol/ml. compared with blank control group, Polyphyllin I and Sorafenib can inhibit S period, blocked the cells in G0/G1 phase, can obviously promote the cell apoptosis. There was a significant difference compared with the blank control group （P〈0.01）. Conclusion Polyphyllin I can significantly inhibit the proliferation of MHCC97H and promote its apoptosis.