以三苯甲烷染料酸性品红作探针检测罐头肉中的痕量Hg

Detection of trace mercury in canned meat using acid triphenylmethane dye fuchsin acid as the probe

建立了检测肉罐头中有毒重金属元素Hg的酸性品红共振光散射法,研究了共振光散射的光谱特征、适宜反应条件、共存物质的影响及反应机理。在pH 6.56的Tris-盐酸缓冲介质中,溴代十六烷基吡啶增敏酸性品红并与Hg(Ⅱ)结合生成三元复合物,使体系的共振光散射(RLS)显著增强并产生新的RLS光谱,在最大共振散射峰附近,Hg(Ⅱ)的质量浓度在0.005~0.40 mg/L与体系的共振光散射增强强度的绝对值│ΔIRLS│呈线性关系,定量限为0.032 mg/kg。方法简便、灵敏,加标回收率为98.5%~102%,相对标准偏差RSD(n=5)为2.0%~2.5%。适于罐头类肉食中Hg的测定。

A new acid fuschin resonance light scattering method for the determination of toxic heavy metal ele- ment mercury in canned meat was established. The spectrum characteristics of resonance light scattering and optimum reaction conditions and the effects of coexistent substances and reaction mechanism of ternary complex were discussed. In the Tris-Hydrochloric acid buffer medium of pH 6.56, cetylpyridine bromide sensitization acid fuschin were com- bined with mercury（ Ⅱ ） to form ternary complexes, which distinctly enhanced the resonance light scattering （RLS） of the system and created a new resonance light scattering spectrum. In the vicinity of the maximum resonance light scattering peak, the absolute value of resonance light scattering enhance intensity （ ｜△IRLs｜） was directly proportion to the mass concentration of mercury（ Ⅱ ） in the range of 0. 005 to 0.40 mg/L with quantitative limitation of 0. 032 mg/kg. The method was simple and sensitive, the standard addition recovery and RSD% （n = 5） were in the ranges of 98.5% - 102% and 2.0% - 2.5% respectively. The method is suitable for the determination of mercury in canned meat.