转录组学在抑菌机制中的应用研究进展

Applications of transcriptomics in research of antimicrobial mechanism

微生物是导致食品腐败变质和引起食源性疾病的主要因素,如何抑制微生物的生长是食品及医药领域的一个重要研究方向,因此,对抑菌物质抑菌机制的研究也已成为目前国内外研究的热点问题。利用转录组学技术可以从转录组水平上揭示各种抑菌物质抑制微生物生长的分子机制。文中综述了转录组学主要的研究方法,介绍了目前应用最多的RNA测序技术(RNA sequencing,RNA-seq)的技术特点以及转录组学在抑菌机制研究中的国内外研究现状。随着高通量测序技术的迅速发展,转录组学在抑菌机制研究中将具有更广阔的应用前景。

Since microorganism is one of the most primary factors to cause food spoilage and foodborne disease, the inhibition of the growth of microorganism is an important research direction in food and medical field. Therefore, the study on antimicrobial mechanism is a hot topic in domestic and aboard academe. The utilization of transcriptomics could reveal the molecular mechanism of various antimicrobial substances against microorganism. The main research methods for transcriptomics, especially the technical features of the most widelyused RNA sequencing technology were reviewed in this paper. The research situations of transcriptomics for antimicrobial mechanism at home and aboard were introduced. With the development of high-throughput sequencing technology,transcriptomics will have extensive application prospects in research of antimicrobial mechanism.