天降血栓通丸早期干预NF-κB信号通路对糖尿病大鼠大血管病变的影响

Effect of early intervention of Tianjiang Xueshuantong pill on NF-κB signal pathway of macroangiopathy in type 2 diabetic rats

目的:研究天降血栓通丸早期干预的方法能否减少实验大鼠主动脉NF-κB蛋白因子及其下游相关细胞因子的表达,进而延缓糖尿病大血管病变的发生发展。方法:将90只wistar大鼠随机分为空白组(15只),造模组(75只),造模组随机分为模型组,天降血栓通丸早期组、中期组、晚期组与盐酸吡格列酮组,予药物干预,观测血糖、MCP-1、MMP-9,NF-κB。结果:与模型组相比,天降血栓通丸各组血糖无明显差异(P>0.05);天降血栓通丸早期、中期给药组与模型组比较,MCP-1降低,差别具有统计学意义(P<0.05);天降血栓通丸早期给药组与西药组比较,MCP-1表达降低,差别具有统计学意义(P<0.05);与模型组相比,天降血栓通丸早期、中期给药组NF-κB及MMP-9蛋白表达下调明显(P<0.05),其中天降血栓通丸早期组下调最显著。结论:天降血栓通丸早期干预有效降低NF-κB调控的MCP-1及MMP-9蛋白水平表达,可能是其延缓2型糖尿病大鼠大血管病变发生发展的重要机制之一。

Objective： To research whether the early intervention of pill can reduce the expesssions of experi- mental rat＇s aortic NF - kappa B protein factor and its related downstream cytokines, in order to delay the occur- rence and development of diabetic macroangiopathy. Methods： 90 Wistar rats were divided randomly into control group （15 rats）, modeling group （75 rats）. The modeling group was divided again randomly into model group, early - , mid - , and late - term intervenient group of pill , pioglitazone hydrochloride group. Given drug for inter- vention, blood glucose, monocyte chemotactic protein - 1 （ MCP - 1 ）, matrix metalloproteinases - 9 （ MMP - 9 ）, nuclear factor - kappa B （ NF - κB） were observed. Results ： Compared with the model group, the blood glucose of early - , mid - , and late - term intervenient group of pill had no significant difference （ P 〉 0.05 ） ; compared medicine group, the MCP - 1 expression decreased, the difference was statistically significant （P 〈 0. 05 ） ; com- pared with the model group, the protein expressions of NF - KB and MMP - 9 of early - , and mid - term interven- ient group of pill were lowered obviously（P 〈 0. 05）, and among them, it is the most obvious in early -term in- tervenient group of pill. Conclusion： The early intervention of pill can effectively reduce the expression of MCP - 1 and MMP - 9 protein regulated by NF - kappa B, which may be one of the important mechanisms to retard the occurrence and development of maeroangiopathy in type 2 diabetic rats.