高效液相色谱分析虫草中核苷类成分

Determination of Nucleosides in Caterpillar Fungi by High Performance Liquid Chromatography

为虫草中核苷类成分研究和质量控制提供参考,建立了一种高效液相色谱(HPLC)定量分析胞苷酸、尿苷酸、尿嘧啶、鸟苷酸、肌苷酸、次黄嘌呤、尿苷、腺苷酸、胸腺嘧啶、腺嘌呤、2′-脱氧腺苷酸、肌苷、鸟苷、2′-脱氧鸟苷、胸苷、腺苷、2′-脱氧腺苷、虫草素和N6-(2-羟乙基)腺苷这19种核苷的方法。采用X select HSS T3 column(5μm,4.6 mm×250 mm)色谱柱,流动相为乙腈和水,梯度洗脱,流速1 m L/min,柱温40℃,检测波长254 nm。研究表明:19种核苷精密度RSD为0.11%~2.82%,稳定性RSD为0.22%~1.63%,保留时间RSD为0.14%~3.47%,重复性RSD为1.3%~5.2%,回收率为84.9%~108.3%,说明该方法准确、可靠,采用该方法成功测定了多种虫草产品中核苷的质量分数。

In order to afford a reference for the study of nucleosides and quality control of caterpillar fungi products.a method for simultaneous determination 19 kinds of nucleosides using HPLC was established including CMP, UMP, uracil, GMP, IMP, hypoxanthine, uridine ,AMP, thymine, adenine, dAMP,inosine.guanosine,2＇-deoxyguanosine,thymidine,adenosine,2＇-deoxyadenosine,cordycepin, N6- （2-hydroxyethyl）-adenosine. Chromatographic conditions： X select HSS T3 column （5μm,4.6 mm×250 mm）,acetonitrile and water as mobile phase with gradient elution, flow rate 1 mL/min, colunm temperature 40 ℃,detection wavelength 254 nm. Methodological research showed this wasan accurate and reliable method which RSD of precision ranged 0.11%-2.82% ,RSD of stability ranged 0.22%-1.63%, RSD of retention time ranged 0.14%-3.47%, RSD of reproducibility ranged 1.3%-5.2% ,and recovery ranged 84.9%-108.3%. The nucleoside content in some caterpillar fungi products were successfully determined using this method.