改良枕大池二次注血法建立大鼠蛛网膜下腔出血模型

The SAH Model by Improved Two Times Injections of Arterial Blood Solvate into Cisterna Magna Directly in Rats

目的:建立大鼠蛛网膜下腔出血模型,通过研究其出血后认知、行为学改变,基底动脉变化探讨枕大池穿刺二次注入自体动脉血溶血物法建立大鼠蛛网膜下腔出血模型的实际可行性。方法:将72只清洁级雄性SD大鼠(体重300~350 g)随机分成对照组(n=12只)、实验组(n=60只)。实验组又分别划分为出血后12 h、24 h、48 h、3 d、5 d 5个时相组,每个时相组各12只大鼠。造模后,通过水迷宫试验观察各时相点大鼠蛛网膜下腔出血后的认知、行为学改变,评价大鼠的学习记忆功能障碍。结果:水迷宫结果显示实验组大鼠与正常对照组比较,逃避潜伏期明显延长,跨越平台次数减少,差异均有统计学意义(P<0.05)。大体标本观察,对照组脑蛛网膜下腔未见出血,实验组大鼠蛛网膜下腔出血明显,可见有大量血液或血凝块弥漫分布于蛛网膜下腔。结论:枕大池穿刺二次注入自体动脉血溶血物法建立模型操作简便易行,可重复性强,大鼠学习记忆功能障碍改变明显。大鼠基底动脉痉挛明显,模型复制成功。

Objective： To study the practical feasibility of two times injections of arterial blood solvate into eisterna magna directly by establishing SAH model in rats by observating the cognitive and behavioral change after subaraehnoid hemorrhage in rats.Method： 72 clean-level male SD rats were randomly divided into control group（ n=12 ） and experimental group （ n=60 ） .Experimental group were randomly divided into 12 h, 24 h, 48 h, 3 d, 5 d group, 12 rats in each group.After modeling, the cognitive and behavioral changes of subaraehnoid hemorrhage in rats at different time points by water maze test were observed, the learning and memory impairment in rats were evaluated.Result： Morris water maze showed that the escape latency was prolonged significantly and the number of cross-plalform reduced in each group of SAH compared with those of experimental group, the differences were statistically significant （ P〈0.05 ） .In general observation, there was no hemorrhage in the subarachnoid space of the control group.The subarachnoid hemorrhage in the experimental group was obvious, and there was a large amount of blood or blood clot distributed around the subaraehnoid space.Conclusion： It is easy to prform and repeat the SAH model in rats established by two times injections of arterial blood solvate into eisterna magna directly, whose clinical symptoms and the dysfunction of learning and memory are changed significantly.The artery cramps obviously, which meant the model is established successfully.