卵巢上皮性癌组织骨桥蛋白和CD133表达及意义

EXPRESSION AND SIGNIFICANCE OF OSTEOPONTIN AND CD133 IN TISSUES OF OVARIAN EPITHELIAL CARCINOMA

目的探讨骨桥蛋白(OPN)和CD133在卵巢上皮性癌组织中的表达及其意义。方法采用免疫组织化学方法检测OPN和CD133在卵巢癌、卵巢交界性肿瘤、卵巢良性肿瘤及正常卵巢组织中的表达。结果卵巢癌组织中OPN和CD133的阳性表达水平与正常卵巢组织、卵巢良性肿瘤组织、卵巢交界性肿瘤组织比较,差异有显著性(χ~2=23.45、35.96,q=-8.24^-3.82,P<0.05),正常卵巢组织中OPN和CD133的阳性表达水平与良性肿瘤组织比较,差异无显著性(P>0.05)。OPN、CD133阳性表达与卵巢癌临床分期、病理分期及是否淋巴结转移有关(χ~2=4.19~12.10,P<0.05),而与卵巢癌组织学类型无关(P>0.05);卵巢组织中OPN与CD133的表达呈正相关关系(r=0.525,P<0.05)。结论 OPN与CD133的协同作用可能对卵巢癌进展起重要作用,二者在卵巢癌组织中表达的关系,可为临床诊治该病提供指导意义。

Objective To investigate the expression and biological significance of osteopontin （OPN） and CD133 in the tissues of ovarian epithelial carcinoma. Methods Immunohistochemistry was used to measure the expression of OPN and CD133 in tissues of ovarian cancer, borderline ovarian tumor, benign ovarian tumor, and normal ovary. Results The positive expres sion levels of OPN and CD133 in ovarian cancer tissues were significantly different from those in tissues from normal ovary, benign tumor, and borderline ovarian tumor （Z2：23.45,35.96;q= 8.24----3.82~P^0.05）. However, the positive expression levels of OPN and CD133 in normal tissues were not significantly different from those in benign tumor tissue （P〉0.05）. Positive expression of OPN and CD133 was correlated with clinical stage of ovarian cancer, degree of tumor differentiation, and lymph node metastasis （χ2 =4.19--12.10,P〈0.05）, and had nothing to do with the histological type of ovarian cancer （P〉0.05）. In ovarian tissue, the expression of OPN was positively correlated with CD133 expression （r=0.525,P〈0.05）. Conclusion The synergistic effect between OPN and CD133 may play an important role in ovarian cancer development. The relationship between their expression in ovarian cancer tissue can provide a guidance for the clinical diagnosis and treatment.