银杏叶提取物EGb761对氧化应激下白癜风黑素细胞抗氧化作用的影响

Effects of ginkgo biloba extract EGb761 on anti-oxidation of melanocytes under oxidative stress in vitiligo

目的:明确银杏叶提取物(EGb761)对氧化应激下白癜风黑素细胞抗氧化作用的影响。方法:人正常黑素细胞系PIG1予以H2O2处理建立氧化应激模型,予以不同浓度(50μg/m L、100μg/m L、200μg/m L、300μg/m L、400μg/m L)EGb761处理,采用MTT法检测PIG1细胞活力,生物化学方法检测脂质过氧化代谢产物丙二醛(MDA)、乳酸脱氢酶(LDH),谷胱甘肽过氧化物酶活性及含量,流式细胞仪检测细胞内活性氧(ROS)水平。结果:与模型组比较,EGb761组PIG1细胞活力、SOD、GSHPx水平增高,ROS、MDA及LDH的表达水平降低。结论:银杏叶提取物EGb761可保护黑素细胞抵抗氧化应激损伤。

Objective： To determine the effect of ginkgo biloba extract（EGb761） on the anti-oxidation of melanocytes under oxidative stress in vitiligo. Methods： The normal human melanocyte cell line PIG1 was treated with H_2O_2 firstly,followed by different concentrations（ 50 μg/m L,100 μg/m L,200 μg/m L,300μg/m L,400 μg/m L） of EGb761. The cell viability was measured by MTT. The levels of lipid peroxide metabolites such as malondialdehyde（ MDA）,lactic dehydrogenase（ LDH） and GSH-Px were measured by biochemical methods. The level of cellular reactive oxygen species（ ROS） was detected by flow cytometry. Results： Compared with control group,the expression levels of PIGI cell viability,SOD and GSH-Px were increased,and the expression levels of ROS,MDA and LDH were decreased in EGb761 group. Conclusion：EGb761 has a potential role in protecting melanocytes against H_2O_2-induced oxidative stress.