Smad7对肝癌细胞增殖和迁移的影响及其临床意义

The Role of Smad7 in Hepatocellular Carcinoma Proliferation and Migration and Its Clinical Significance

目的:探究Smad7对肝癌细胞增殖和迁移的影响及其临床意义。方法:通过转染pcDNA3.1(+)-Smad7质粒或Smad7的小干扰RNA使得Smad7在肝癌细胞系HepG2和Huh7中过表达或敲减,应用MTT法检测Smad7对肝癌细胞增殖的影响,采用细胞划痕实验以及Transwell实验探讨Smad7对肝癌细胞迁移的影响。采用qRT-PCR检测9例肝癌癌患者手术切除的组织样本中Smad7的表达。结果:过表达Smad7的肝癌细胞增殖能力与对照组相比有明显的下降,而敲减smad7能够促进肝癌细胞的增殖。过表达smad7的肝癌细胞穿过Transwell小室底膜的能力显著下降,而敲减Smad7能够促进这种能力。Smad7在肝癌癌旁组织中的表达显著高于癌组织。结论:Smad7能够在肝细胞肝癌的进展中发挥负向调控作用。

Objective： To investigate the role of Smad7 in the Hepatocellular carcinoma （HCC） migration and proliferation and its clinical significance. Methods： Through transfecting pcDNA3.1 （＋）-Smad7 or siRNA Smad7 to overexpress or knockdown the Smad7 expression in HCC cell lines HepG2 and Huh7. The MTT assays were used to test the role of Smad7 in proliferation of HCC cells. Transwell and wound-healing assays were used to detect the effect of Smad7 on migratory ability in both tow cell lines. RT-PCR was used to test the Smad7 expression in 9 clinical HCC patients＇ specimens. Results： As the results, overexpression of Smad7 significantly inhibited the proliferation of cells compared with the control group, while knockdown Smad7 promoted the proliferation. At the same time, overexpression of Smad7 could inhibit the migratory ability of HCC cells compared with the control group, while knockdown smad7 could accelerate this ability. The expression of Smad7 in cancer tissue was significantly lower compared with normal mucosa tissue adjacent to cancer. Conclusions： Smad7 is a kind of anti-progressive molecule in HCC.