SHIP1在急性髓细胞白血病患者中的表达及对人白血病细胞凋亡的影响研究

Expression of SHIP1 in the Patients with Acute Myeloid Leukemia and Its Influence on the Apoptosis of Human Leukemia Cells

目的:探讨含SH2结构域的肌醇1(SHIP1)在急性髓细胞白血病患者中的表达及对人白血病细胞凋亡的影响。方法:采用Western blot检测收集的急性髓细胞白血病患者骨髓中SHIP1的表达。人白血病细胞U937转染SHIP1过表达载体(pEGFP-SHIP1组)及对照空载体(pEGFP组),同时设置对照组,对照组细胞不转染载体,其他步骤同pEGFP-SHIP1组和pEGFP组。流式细胞仪检测48 h的细胞凋亡情况,Western blot检测48 h细胞中SHIP1、Bcl-2、Bax、Akt、p-Akt的表达。结果:急性髓细胞白血病患者骨髓中SHIP1表达明显低于正常人(P<0.05)。pEGFP-SHIP1组细胞中SHIP1、Bax表达和凋亡率均明显高于pEGFP组及对照组(P<0.01),Bcl-2、p-Akt表达均明显低于对照组(P<0.01)。结论:SHIP1在急性髓细胞白血病患者骨髓中表达下调,其可能通过Akt信号促进人白血病细胞凋亡。

Objective： To investigate the expression of SHIP1 in the patients with acute myeloid leukemia and its effect on the apoptosis of human leukemia cells. Methods： The expression of SHIP1 in the bone marrow of patients with acute myeloid leukemia was detected by Western blot. U937 cells was transfected with SHIP1 expression vector （pEGFP-SHIP1 group） and empty vector control （pEGFP group） respectively, U937 cells without transfection were used as the control group. Flow cytometry was used to detect the apoptosis of the cells, the expression of SHIP 1, Bcl-2, Bax, Akt, p-Akt were detected by western blot. Results： The expression of SHIP 1 in the bone marrow of patients with acute myeloid leukemia was significantly lower than that of the normal human bone marrow SHIP1 （P〈0. 01）. The SHIP1 and Bax expressions as well as the apoptotic rate ofpEGFP-SHIP1 group were significantly higher than those of the control group（P〈0.01）, while the Bcl-2 and p-Akt expressions were significantly lower than those in the control group（P〈0.01）. Conclusions： SHIP1 expression was down regulated in the bone marrow of patients with acute myeloid leukemia. SHIP1 could promote the apoptosis of human leukemia cells via Akt signaling pathway.