针刀干预对帕金森病模型大鼠小胶质细胞及IL-1β表达的影响

Effects of Acupotomy on Microglic and the Expression of IL-1Β in Parkinson's Diseases

目的:探讨针刀疗法治疗帕金森病(Parkinson's Disease,PD)的炎性作用机制。方法:将48只大鼠随机分为正常组、模型组、针刀组、电针组。采用立体定位技术将6-羟基多巴胺(6-DAOH)注入大鼠右侧纹状体制备PD大鼠模型。针刀组于枕下肌群和C1-2横突处进行松解干预,2次/周,连续4周;电针组选"百会""太阳"穴,由"百会"透刺"太阳"穴进行电针干预,3次/周,连续4周。采用HE染色光镜下观察神经元细胞形态、数目及小胶质细胞数目变化,酶联免疫吸附法(ELisa)检测IL-1β的水平。结果:与正常组比较,模型组大鼠损毁侧多巴胺能神经元细胞数目减少,小胶质细胞数目增多,IL-1β水平升高,差异有统计学意义(P<0.05);与模型组比较,针刀组和电针组大鼠损毁侧多巴胺能神经元细胞数目增多,小胶质细胞数目减少,IL-1β水平降低,差异有统计学意义(P<0.05)。结论:针刀疗法可以改善多巴胺能神经元细胞的形态和数目,其机制可能是通过抑制小胶质细胞激活介导的炎性反应,降低炎性因子IL-1β的含量,从而治疗PD。

Objective： To explore the mechanism of acpotomy in Parkinson′s disease （PD）.Methods： Fourty-eight rats were randomly divided into a model group, a normal group, an acupuncture group and an acupotomy group.Using the technology of Stereo-positioning to inject the 6-hydroxy dopamine （DAOH） into the right striatum of rats to build PD rats model;Rats in the acupotomy group were treated by disposable acupotomy at suboccipital muscle group and transverse process of C1-2, twice a week for continued four weeks.Electroacupuncture （EA） group were treated by acupuncture at Baihui and Taiyang, to strike Taiyang from Baihui, third a week for four weeks.HE staining method was used for observation of morphology, quantity of neurons and quantity of microglia, and Elisa method was used for testing the expression of IL-1β.Results： Compared with the nomal group, the quantity of the dopaminegic neurons in the damaged side of PD model group obviously decreased, while microglic increased, and the level of IL-1β was improved with significant difference（P〈0.05）.Compared with the model group, the quantity of the dopaminegic neurons in the damaged side of the acpotomy and EA group increased obviously, while microglic decreased, and the level of IL-1β was alleviated with significant difference（P〈0.05）.Conclusion： Acupotomy can improve the morphology and quantity of dopaminergic neurons and its mechanism may be mediated by inhibition of microglia activation of inflammatory reaction, reducinf the content of inflammatory cytokines IL-1 beta, thus to treat PD.