益气解毒方对ACHN人肾癌干细胞微环境影响的实验研究

Effects of Yiqi Jiedu Fang( 益气解毒方) on Microenviroment of ACHN Human Renal Carcinoma Stem Cells

目的:观察益气解毒方对人肾癌干细胞微环境的影响,探讨其抗肿瘤机制。方法:建立ACHN人肾癌干细胞裸鼠皮下移植瘤模型,随机分为对照组(生理盐水)和益气解毒方组,连续给药25 d,剥取瘤体计算瘤重及抑瘤率;免疫组织化学方法测定移植瘤组织血管内皮生长因子(VEGF)、转化生长因子-β1(TGF-β1)、基质金属蛋白酶-2(MMP-2)、乏氧诱导因子-1α(HIF-1α)表达。结果:与对照组比较,益气解毒方组移植瘤重量显著下降,抑瘤率为37.1%(P<0.01);益气解毒方组瘤组织VEGF、TGF-β1、MMP-2、TGF-β1蛋白表达的平均光密度值降低(P<0.05)。结论:益气解毒方能够抑制ACHN人肾癌干细胞移植瘤的生长,对肾癌干细胞血管生成环境、细胞外因子环境、细胞外基质环境、乏氧环境等微环境的影响是其抗肿瘤作用机制之一。

Objective： To clarify the effects of Yiqi Jiedu Fang on the microenvironment of human renal car- cinoma stem cells. Methods： The model of ACNH - transplanted tumor was established in nude mice. The mice were randomly divided into control group and Yiqi Jiedu Fang group, the medication lasted for 25 days. The tumor weight and the tumor inhibition rate were calculated. The expressions of VEGF, TGF - f31, MMP -2 and HIF - 1 et were detected by immunohistochemical assay. Results： Compared with the control group, transplanta- tion tumor weight of Yiqi Jiedu Fang group was significantly decreased（P 〈0. 01 ）. The light density value of VEGF,TGF - 61, MMP - 2 and HIF - let expressions in ACHN - transplanted tumor of the Yiqi Jiedu Fang group were significantly decreased（P 〈 0. 05 or P 〈 0. 01 ）. Conclusion： Yiqi Jiedu Fang can inhibit the growth of ACHN- transplanted tumor, inhibiting VEGF,TGF-β1, MMP- 2 and HIF- 1α expressions is one of the mechanisms of its anti - tumor effect. Key words