基于转录组的芒果MYB家族基因的鉴定及分析

Identification and Analysis of MYB Transcription Factor Family Based on Transcriptome Data in Mango(Mangifera indica L.)

MYB家族作为植物中较大的转录因子家族之一,参与调控植物的多种生理活动。本研究基于芒果果实转录组测序结果,鉴定出71个MYB家族蛋白,其中包含1个4R-MYB蛋白、3个R1R2R3-MYB蛋白、60个R2R3-MYB蛋白和7个MYB相关蛋白。进化树及基序分析表明:除个别蛋白外,相同类型的MYB蛋白均聚在一起,且相近分支的MYB蛋白具有相同或相似的基序。60个全长MYB家族蛋白中,大部分为不稳定蛋白,且均为不含跨膜结构和信号肽的亲水性蛋白,亚细胞定位分析均定位于细胞核。进化树分析发现芒果R2R3-MYB蛋白与拟南芥有较高的保守性。R2R3-MYB蛋白保守域分析发现,R2和R3结构域均有多个氨基酸保守不变。GO分析发现芒果R2R3-MYB蛋白共注释到生物学过程、细胞组分和分子功能3大类功能的15个亚类。

As one of the large transcription factor families, MYB family is involved in regulating a variety of physiological processes in plants. Based on the results of transcriptome sequencing of the fruits in mango, 71 sequences of MYB family proteins, including one 4R-MYB protein, three R1R2R3-MYB proteins, 60 R2R3-MYB proteins and 7 MYB related proteins, were identified in the study. The phylogenetic tree and motifs analyses showed that, with a few individual exceptions, MYB proteins of the same type clustered together, and the majority of the close members in the phylogenetic tree exhibited same or similar motifs compositions. 60 full-length MYB proteins were analyzed, in which most were unstable, and all of those proteins were hydrophilic proteins, without a signal-peptide and transmembrane region. Subcellular localization analysis indicated that those proteins were located in nucleus. In addition, the phylogenetic analyses of the mango（ 60 members） and Arabidopsis（ 126 members） R2R3-MYB proteins showed that those proteins were highly conserved between mango and Arabidopsis.Conserved domain（ R2 and R3 repeats） of mango R2R3-MYB proteins contained multiple conserved amino residues. GO（Gene Ontology） annotation showed that those proteins could be categorized into 15 function subgroups of three main categories： biological processes, cellular components and molecular function.