利用SSR分子标记研究白菜类亚种资源的遗传多样性

Genetic Diversity Analysis in Chinese Cabbage [ssp. chinensis(L.) Makino] Resources Based on SSR Molecular Markers

为研究几种白菜类亚种资源的演变过程,利用白菜SSR引物对94份白菜类亚种种质资源进行遗传多样性分析。结果表明,19对SSR引物扩增出137个条带,平均每对引物可扩增出7.16个;多态性位点百分率(P)、基因杂合度(He)、有效等位基因数(Ne)、Shannon-Wiener指数(H’)和多态信息含量(PIC)的均值分别为:99.9%、0.897、11.598、2.454、0.886,除P值外,4个指标间相关性极显著;94份白菜类亚种种质资源的UPGMA聚类结果表明:在遗传相似系数为0.58时实验材料分为3类,多数地方种菜心与小白菜亲缘关系更近。白菜SSR引物对大(小)白菜基因组具有较好的多态性、红菜苔次之、野生芥菜最低。

In order to analyze the evolution of Chinese cabbage [ssp. chinensis（L.） Makino] resource, a total of94 Chinese cabbage [ ssp. chinensis（ L.） Makino] materials were used to assess the genetic diversity. The results showed that, nineteen cabbage SSR primers combinations amplified 137 alleles, 7.16 loci on average for each pair of primers. The average of polymorphism loci percentage（ P）, genetic heterozygosity（ He）, effective number of alleles（Ne）, Shannon-Wiener index（ H＇） and polymorphism information content（ PIC） was 99.9%, 0.897, 11.598,2.454, 0.886, respectively, and there were extremely significant correlation among them except P value. 94 Chinese cabbage [ssp. chinensis（L.） Makino] materials were clustered into three groups by UPGMA when GSC was 0.58,much of local Chinese cabbage was closer relationships with pakchoi. The order of genome amplification polymorphism by Chinese cabbage SSR primers was（small） cabbage, red bolt second lowest and wild mustard.