清瘟败毒饮对大鼠急性腹膜炎炎性因子的影响

Effect of Qinwenbaiduyin Decoction on Inflammatory Factors in Rats with Acute Peritonitis

目的探讨中医温病经典名方清瘟败毒饮在治疗盲肠穿孔急性腹膜炎脓毒血症时对急性炎性因子的影响。方法将96只成年SD大鼠适应性饲养1周后随机分为4组,即对照组(A组)、盲肠结扎穿孔组(CLP模型组)、清瘟败毒饮低剂量组(B组)及清瘟败毒饮高剂量组(C组)。实验组分别于模型制备后12、24、36、48h给SD大鼠用生药4ml灌胃(低剂量组30.8g/kg,高剂量组40.8g/kg)。对照组及CLP模型组在相同时间点给予等量生理盐水4ml。各组大鼠在相应时间点处死取材。并留取血液标本。进行血清IL-8、IL-10、TNF-α检查。结果 C组12、24、36、48h的血液标本中TNF-α、IL-8明显低于B组及CLP模型组;而IL-10明显高于B组及CLP模型组,差异有统计学意义。结论 C组实验室资料均明显优于CLP模型组及B组,清瘟败毒饮在盲肠穿孔、急性腹膜炎、脓毒血症时对急性炎性因子具有正面的影响效果。

Objective To investigate antipyretic disease, pharmacology research of Qinwenbaiduyin. Methods Totally 96 adult experimental SD rats were randomly divided into four groups a week after, control group （group A） , CLP model group, low dose group （group B）, high dose group（ group C）. Experimental group respectively got at 12h, 24h, 36h, 48h decoction by gastric method （low dose group 30.8g/kg and high dose group 40.8g/kg）. The same amount（4ml） of normal saline were given in control group and CLP model group in the same phase. Executed based groups of rats in the corresponding time points. And the blood specimens were taken, and serum IL - 8, IL - 10, TNF - α were checked. Results At 12h, 24h, 36h, 48h, the laboratory data in group C were significantly better than the CLP model group and group B in IL - 8, IL - 10, TNF - α. It had obvious differences. Conclusion Qinwenbaiduyin for gastrointesti- nal perforation, acute peritonitis, sepsis has good therapeutic effect.