莱牧1号抗旱生理响应及灰色关联度分析（英文）

Grey Correlation Analysis of Physiological Traits Related to Drought Tolerance in Pennisetum sp.

以莱牧1号幼苗为实验材料,在不同浓度PEG-6000干旱胁迫条件下,测定幼苗叶片的叶绿素含量、叶片相对含水量、渗透液相对电导率、丙二醛含量、脯氨酸含量、可溶性多糖含量、可溶性蛋白含量的变化,并通过灰色关联度分析各指标与莱牧1号抗旱性的关系。结果表明:随着PEG-6000浓度的提高,叶绿素、叶片相对含水量、相对电导率、可溶性蛋白含量下降,与胁迫质量浓度呈显著负相关;可溶性多糖含量升高,丙二醛含量呈先增加后降低的趋势,脯氨酸含量先降低后升高,且三项指标变化均与胁迫质量浓度呈显著正相关。各生理指标与抗旱指数的关联度依次为:叶片相对含水量(0.980 8)、叶绿素含量(0.967 9)、丙二醛含量(0.876 0)、可溶性多糖含量(0.839 5)、可溶性蛋白含量(0.827 5)、叶片渗透液相对电导率(0.670 8)、脯氨酸含量(0.571 3)。

The drought resistance of Pennisetum sp. was analyzed under different drought stress conditions simulated by various concentrations of PEG-6000. Changesofa series of indexes such as chlorophyll content, water content, relative electrical conductivity of osmotic solution, MDA content, proline content, soluble polysaccharide content and soluble protein content in the leaves were measured.The correlation of these indexes with the drought resistance of Pennisetum sp. was an- alyzed by the method of grey correlation analysis. The results showed that the chlorophyll, water content, relative electrical conductivity and soluble protein con- tentexhibited obvious negative correlation with the stress concentration, which de- creasedfollowing the increase of PEG-6000 concentration. The soluble polysaccha- ride contentshowed an increasing trend,the MDA content showed a rise at first fol- lowed by a declinetrend, while the prolinecontent was just opposite to MDA. The changes of the 3 indexes showed obvious positive correlation with thestress con- centration. The correlation of these indexes with the drought resistance of Pennise- tum sp. were ranked as follows： leaf water content （0.980 8）, chlorophyll content （0.967 9）, MDA content （0.876 0）, soluble polysaccharide content （0.839 5）, soluble protein content（0.827 5）, relative electrical conductivity of osmotic solution（0.670 8）, and proline content（0.571 3）.