人参皂苷Rb_1后处理对大鼠全脑缺血再灌注后认知功能的影响

Effect of Ginsenoside Rb_1 Postconditioning on Cognitive Function after Global Cerebral Ischemia Reperfusion in Rats

目的探讨人参皂苷Rb_1后处理对大鼠全脑缺血再灌注后认知功能的影响及其可能机制。方法 90只SD雄性大鼠随机分为假手术组、模型组和治疗组。治疗组大鼠造模后连续7d给予腹腔注射20mg/(kg·d)人参皂苷Rb_1。模型采用四血管阻断全脑缺血模型。最后一次人参皂苷Rb_1腹腔注射后24h每组各取5只,左侧大脑取海马检测髓过氧化物酶(MPO)活力和NF-κB的DNA结合活力。其余每组各25只大鼠分别于再灌注后第7日开始进行Morris水迷宫实验。结果模型组MPO活力、NF-κB的DNA结合活力、平均潜伏期及探索实验中在第2象限时间比与假手术组比较差异有统计学意义(P<0.05);治疗组与模型组比较,MPO活力和NF-κB的DNA结合活力下降,平均潜伏期缩短,第2象限时间比增高(P<0.05)。结论人参皂苷Rb_1后处理可减轻大鼠全脑缺血再灌注后海马区炎症反应,改善认知功能。

OBJECTIVE To study the effect of ginsenoside Rb： postconditioning on cognitive function in rats with global cerebral isehemia-reperfusion injury so as to investigate its probable mechanism. METHODS Ninety SD male rats were randomly divided into sham operated group, model group, and ginsenoside Rb1 postconditioning group （20rag/（ kg·d） ）. The animals were intraperitoneally administered with drugs for 7 days. Acute injury models with 10-minute ischemia and persistent reperfusion were blocked with Pullsinelli-4 vessel occlusion. Twenty-four hours af- ter the end administration, five rats in each group were killed to obtain their brains. The activity of MPO and NF-κB in the left brain were measured. In the other 25 rats in each group, Morris water maze test was employed to assess spatial learning and memory respectively seven days after reperfusion. RESULTS The above mentioned indexes in the model group showed differences compared with those in the sham operated group （P 〈 0.05 ）. Compared to the model group,the activity of MPO and NF-κB in the brain tissue, the mean escape latencY decreased, and the percent of time course in the second quadrant increased in ginsenoside Rbl postconditioning group （P 〈 0.05 ）. CONCLUSION Ginsenoside Rb1 could relieve the abnormity of inflammatory reaction in the brains, exert protective effect on cognitive impairment in global cerebral ischemia reperfusion injury rats.