地肤子大孔树脂不同洗脱部位的抗氧化活性研究

Antioxidant activity of diferent macroporous resin elution fractions from kochia scoparia. in vitro

目的研究地肤子不同大孔树脂洗脱部位的抗氧化活性。方法地肤子70%乙醇提取物上AB-8型大孔树脂,分别用水和浓度分别为30%、50%、70%、90%的乙醇进行洗脱,得到5个不同洗脱部位（DFZ1、DFZ2、DFZ3、DFZ4、DFZ5）;采用紫外分光光度法测定各部位的总黄酮含量;并通过测定各洗脱部位对·OH和O2-·的清除率和还原力研究其抗氧化活性。结果地肤子不同洗脱部位（DFZ1、DFZ2、DFZ3、DFZ4、DFZ5）的总黄酮含量分别为（0.82±0.02）%、（8.09±0.66）%、（10.31±0.73）%、（2.73±0.09）%、（1.88±0.06）%;其抗氧化活性均随总黄酮浓度的增加而上升。结论地肤子5个洗脱部位均有一定的抗氧化能力。

Objective To investigate the antioxidant activity of diferent ethanol elution fractions on macroporous resin from Kochia scoparia. in vitro. Methods 70% ethanol extract from Kochia Scoparia. was further isolated and purified by AB-8 macroporous resin into the five elution fractions（ DFZ1,DFZ2,DFZ3,DFZ4,DFZ5）,then the total flavonoids of diferent elution fractions were determined by ultraviolet spectrophtometry. The scavenging rate of the five elution fractions to superoxide anion radical and hydroxyl radical and reducing power were determined. Results The total flavonoids contents of DFZ1,DFZ2,DFZ3,DFZ4 and DFZ5 reached（ 0. 82 ± 0. 02） %,（ 8. 09 ± 0. 66） %,（ 10. 31 ± 0. 73） %,（ 2. 73 ± 0. 09） %,（ 1. 88 ± 0. 06） % respectively. The total antioxidant activity increased with the increasing concentration of total flavonoids. Conclusion From the results it could be concluded that the five elution fractions from Kochia Scoparia had strong antioxidant capacityin vitro.