β-tubulin Ⅲ在结肠腺癌组织中的表达及其临床意义

Expression of β-tubulin Ⅲ in colon adenocarcinoma tissue and its clinical significance

目的:检测结肠腺癌患者癌组织中β-tubulinⅢ的表达,探讨其临床意义。方法:收集结肠腺癌组织标本111例,根据β-tubulinⅢ的阳性细胞分布部位分为浸润前沿组(n=72例)和非浸润前沿组(n=39例),采用免疫组织化学法检测结肠腺癌患者癌组织中β-tubulinⅢ的阳性表达率,分析β-tubulinⅢ的表达与患者性别、年龄、肿瘤分化、临床分期、淋巴结转移、复发和死亡等临床病理特征之间的关系。结果:不同性别、淋巴结转移、临床分期、死亡和复发结肠腺癌患者间β-tubulinⅢ的阳性表达率比较差异无统计学意义(P>0.05)。浸润前沿组和非浸润前沿组患者癌组织中β-tubulinⅢ阳性表达率比较差异有统计学意义(χ~2=8.76,P=0.01)。低中分化病例多见于浸润前沿组,而高分化病例多见于非浸润前沿组(χ~2=6.88,P=0.03)。不同分化程度癌组织中β-tubulinⅢ的阳性表达率比较差异有统计学意义(χ~2=5.74,P=0.04)。非前沿组中有无淋巴结转移的患者癌组织中β-tubulinⅢ阳性表达率比较差异有统计学意义(χ~2=6.02,P=0.05)。免疫组织化学法染色,β-tubulinⅢ阳性表达细胞胞质呈黄色颗粒;与低分化组比较,高分化癌组织中β-tubulinⅢ阳性表达细胞明显增多。结论:结肠腺癌组织中β-tubulinⅢ表达与肿瘤的分化程度有关,高分化结肠腺癌组织多见于非浸润前沿组。非浸润前沿组患者β-tubulinⅢ表达与淋巴结转移有关。

Objective.. To detect the expression and clinical significance of β-tubulin Ⅲ in cancer tissue of the patients with colon adenocarcinoma, and to explore its clinical significance. Methods： A total of Ⅲ colon adenocarcinoma tissue samples were obtained. According to the location of β-tubulin Ⅲ positive cells, all patients were divided into front group （n=72） and non-front group （n=39）. The positive expression rate of β-tubulin Ⅲ in the patients with colon adenocarcinoma was detected with immunohistochemistry. The correlations among the expression of β-tubulin Ⅲ and gender, age, tumor differentiation, clinical stage, lymph node metastasis, recurrence and death were analyzed. Results： The expression levels of β-tubulin Ⅲ had no significant differences between the natients with different gerder, age, lymph node metastasis, clinical stages, death and recurrence. The positive expression rates of β-tubulin in cancer tissue of the patients had significant difference between front and non-front groups （X^2 = 8.76, P = 0.01）. Lowly-to-moderately differentiated tissue was more common in front group, and highly-differentiated tissue was more common in non-front group （X^2= 6.88, P= 0.03）. There were significant differences in the expression levels of β-tubulin Ⅲ between cancer tissues with different differentiation degrees （X^22= 5.74, P= 0.04）. In non-front group, lymph node metastasis was closely correlated with the expression of β-tubulin Ill （X^2=6.02, P=0.05）. The results of immunohistochemical staining showed that the β- tubulin Ⅲ positive-expressing cells were colored brown-yellow. The number of cells with positive β-tubulin m expression was significantly increased in highly differentiated tissue compared with low-differentiated tissue. Conclusion： The expression of β-tubulin Ⅲ is closely related to tumor differentiation in colon adenocarcinoma tissue. The highly differentiated colon adenocarcinoma tissue is more common in non-front group in which the expression of β-tubulin Ⅲ is related to lymph node metastasis.