大网膜包裹构建预血管化组织工程骨

Engineering Vascularized Bone with Omentum Wrapping

目的:探讨大网膜包裹构建预血管化组织工程骨的可行性。方法:按文献描述方法制备双相陶瓷骨材料。体外分离培养兔脂肪干细胞并种植于双相陶瓷骨材料构建ADSCs双相陶瓷骨复合物。将复合物包裹于腹腔大网膜异位成骨,并与复合物肌肉包裹对比,于术后4、8、12周通过HE染色及免疫组化观察成骨情况及血管生成情况。结果:成功分离培养脂肪干细胞,复合双相陶瓷骨培养扫描电镜观察证实大量细胞黏附支架材料。术后4、8、12周,腹腔大网膜包裹异位成骨新骨形成面积及血管生成均大于肌肉包裹异位成骨,实验组术后4周可见新骨生成,术后12周新骨生成更加成熟。结论:腹腔大网膜包裹异位成骨优于肌肉包裹异位成骨,腹腔大网膜包裹ADSCs双相陶瓷骨复合物是构建血管化成熟编制骨的一种有效的新方法。

Objective To assess the feasibility and quality of engineering bone packed by the greater omentum.Methods Biphasic ceramic biologic bone was prepared based on the literature. Rabbit adipose-derived stem cells（ ADSCs） were isolated,cultured,and incorporated in biphasic ceramic biologic bone in vitro.The composites（ ADSCs/Biphasic ceramic biologic bone） were observed by scanning electron microscope（ SEM）. Then,the composites were wrapped with omentum in abdomen,and the composites were embedded into the paraspinal muscle as control. Histological quantitative analysis and western blot assay were conducted 4,8 and 12 weeks after implantation. Results ADSCs were isolated successfully and SEM demonstrated integration of ADSCs with the biphasic ceramic biologic bone scaffold. The results of in vivo experiments showed that new bone and vasular formation in experiment group were significantly higher than those in the control group.The newly formed bone and bone metabolism appeared at 4 weeks and newly formed bones were more mature at 12 weeks in experiment group.Conclusion The treatment of hetetotopic osteogenesis by intraperitoneal greater omentum is superior to that of muscle wrapped ectopic osteogenesis. The ADSCs/biphasic ceramic bone complex wrapped by the greater omentum in the abdominal cavity is an effective new method for the establishment of vascularized mature bone.