中药散剂在抗肿瘤转移实验中的研究

Study of Chinese Medicine Power in Anti-Tumor Metastasis Experiment

目的:中药散剂在动物实验中应用较少,本研究重在对比中药散剂和煎剂在肿瘤学常规体内外实验中的差异。方法:根据文献及药物特性制备双参颗粒散剂、煎剂及醇提产物,体外实验对比醇提双参颗粒散剂和传统水煎剂对Lewis肺癌细胞的影响;动物实验观察2种剂型对C57BL/6荷瘤小鼠肺转移的抑制作用。结果:和传统煎剂相比,双参颗粒醇提产物对肿瘤细胞有更明显的抑制增殖及迁移、促进凋亡的作用,其优势在于抑制早期肿瘤生长的作用,对肺转移无显著优势。结论:中药散剂和传统汤剂在肿瘤学常规实验中有较大差别,应进一步深入研究。

Objective：There are few applications of Chinese medicine powder in animal experiments.This study is to compare the differences between the Chinese medicine powder and decoction in oncology experiment in vitro and vivo.Methods：Shuangshen granules power,decoction and alcohol extract were prepared based on the literature and characters of the medicinals.In vitro experiment,the effects of Shuangshen granules power alcohol extract and traditional decoction on lewis lung cancer cells were compared;in vivo experiment,two dosage forms were compared in efficacy on lung metastasis inhibition in C57BL/6 tumor-bearing mice.Results：The powder had more obvious effects on growth/migration inhibitory and apoptosis promotion,which also showed better effects on early tumor-growth inhibition than decoction,but not on lung metastasis.Conclusion：there is great difference between Chinese medicine power and decoction in oncology routine experiment,therefore more research should be done with it.