逍遥散对肝郁脾虚证大鼠胃组织基因表达谱的影响

Effects of Xiaoyao Powder on stomach gene expression profile of rats with liver-depression and spleen deficiency

目的:在全基因组水平上系统探讨逍遥散大、中、小剂量对慢性束缚应激肝郁脾虚证大鼠胃组织基因表达谱的影响及其抗应激调节机制。方法:采用Agilent大鼠全基因组表达谱芯片检测慢性束缚应激肝郁脾虚证大鼠模型(每天束缚3h,连续21 d)正常组、模型组、逍遥散组(大、中、小剂量)胃组织基因表达的差异,筛选出组间两两比较的差异基因表达谱,并利用生物信息学技术对差异基因表达谱进行GO功能分析、信号通路分析。结果:模型组、逍遥散大、中、小剂量组具有不同的差异基因表达谱,多条生物过程和信号通路的功能被显著上调或下调,其中模型组、逍遥散大、中、小剂量组差异基因参与的显著生物过程分别有597、1216、817、924个功能基因群,参与的显著信号通路分别有27、60、41、59条。结论:肝郁脾虚证具有胃组织差异表达基因组学背景,逍遥散大、中、小剂量对肝郁脾虚证大鼠胃组织众多差异表达基因及功能改变的信号通路、生物过程均有一定的干预作用,中剂量结果显示优于大剂量和小剂量。

Objective：To systematically investigate the different effects of large, medium and small dose of Xiaoyao Powder on gene expression profile in stomach tissue of rats with the syndrome of liver-depression and spleen-deficiency induced by chronic im- mobilization stress and to study the anti-stress regulatory mechanism of the three different doses at the whole genome. Methods： The rat whole genome expression chips （Agilent） were used to detect the differences of gene expression in the Stomach of the rat model with the syndrome of liver-depression and spleen-deficiency induced by 21-day chronic immobilization stress（ 3 hours per day） in normal group, model group and Xiaoyao Powder groups （ large, medium and small dose, respectively） , and any differential gene expression profiles between groups were recognized and selected. Then the differential gene expression profiles were analyzed through gene ontology and signal pathway using bioinformatics. Results：The differential gene expression profiles of model group and Xiaoyao Powder groups （ large, medium and small dose） were different. The functions of multiple biological processes and sig- naling pathways were significantly up-regulated or down-regulated. The significantly-changed biological processes participated by differential gene of the model group and the Xiaoyao Powder groups（ Large, medium and small dose） had 597,1216,817,924 function gene groups respectively, and signal pathways had 27,60,41,59 respectively. Conclusion：The syndrome of liver-depres- sion and spleen-deficiency has the background of differentially expressed genomics in stomach. The large, medium and small dose of Xiaoyao Powder all have certain intervention effects on multiple stomach differentially expressed genes, the signal pathways and the biological processes whose functions are both changed. The result shows the medium dose of Xiaoyao Powder is better than the large and small dose.