逍遥散对肝郁脾虚证大鼠下丘脑基因表达谱的影响

Effects of Xiaoyao Powder on hypothalamus gene expression profile of rats with liver-stagnation and spleen-deficiency syndrome

目的:在全基因组水平上系统探讨逍遥散对慢性束缚应激肝郁脾虚证大鼠下丘脑基因表达谱的影响及其抗应激调节机制。方法:采用Agilent大鼠全基因组表达谱芯片检测慢性束缚应激肝郁脾虚证大鼠模型(每天束缚3 h,连续21 d)正常组、模型组、逍遥散组(大、中、小剂量)下丘脑基因表达的差异,筛选出组间两两比较的差异基因表达谱,并利用生物信息学技术对差异基因表达谱进行GO功能分析、信号通路分析。结果:模型组、逍遥散大、中、小剂量组具有不同的差异基因表达谱,多条生物过程和信号通路的功能被显著上调或下调,其中模型组、逍遥散大、中、小剂量组差异基因参与的显著生物过程分别有909、712、516、1322个功能基因群,参与的显著信号通路分别有60、57、41、90条。结论:肝郁脾虚证具有下丘脑差异表达基因组学背景,逍遥散大、中、小剂量对肝郁脾虚证大鼠下丘脑众多差异表达基因及功能改变的信号通路、生物过程均有一定的干预作用,大剂量和中剂量结果显示优于小剂量。

Objective：To systematically investigate the effect of Xiaoyao powder on hypothalamus gene expression profile of rats with syndrome of liver-depression and spleen-deficiency induced by chronic immobilization stress and its anti-stress regulatory mechanism at the whole genome. Methods：The rat whole genome expression chips（Agilent） were used to detect gene expression differences in the hypothalamus of the rat model with liver-stagnation and spleen-deficiency syndrome induced by chronic immobi- lization stress （ daily immobilization stress for 3 h for 21 days） in normal group, model group and Xiaoyao powder group（ large, me- dium and small doses） and any different gene expression profiles between groups were recognized and selected. The hypothalamus gene expression profile was studied through gene ontology and signal pathway analyses using bioinformatics. Results ：The differenti- al gene expression profiles of model group and Xiaoyao Powder groups （large, medium and small dose） were different. The func- tions of multiple biological processes and signaling pathways were significantly up-regulated or down-regulated. The significantly- changed biological processes participated by differential gene of the model group and the Xiaoyao Powder groups （Large, medium and small dose） had909,712,516,1322 respectively, and signal pathways had 60,57,41,90 respectively. Conclusion：The liver- stagnation and spleen-deficiency syndrome has the background of differentially expressed genomics in hypothalamus. The large, medium and snmll doses of Xiaoyao powder all have a certain intervention effect on multiple hypothalamus differentially expressed genes, signal pathway and biological process whose functions are both changed. The result shows the large and medium doses are better than the small dose.