丹参酮ⅡA磺酸钠注射液对兔心肌缺血预处理后血管内皮损伤的保护作用

Protective effect of Danshen A Sodium Sulfonate Injection on Vascular Endothelial Injury after Ischemic Preconditioning in Rabbits with Myocardial Ischemia Preconditioning

目的:通过建立兔急性心肌缺血/再灌注损伤动物模型,观察丹参酮ⅡA磺酸钠注射液对实验动物心肌缺血预处理后血管内皮损伤的保护作用及其对兔心肌酶谱的影响,探讨丹参酮ⅡA磺酸钠注射液对心功能潜在的影响机制。方法:取24只健康兔随机分为缺血/再灌注组、缺血预处理A组、缺血预处理B组,其中其中缺血预处理A组按2.5 m L/(kg·d)、缺血预处理B组按5 m L/(kg·d)耳缘静脉注射丹参酮ⅡA磺酸钠注射液进行缺血前预处理,缺血/再灌注组注射1m L生理盐水,预处理1周后3组动物均采用手术结扎冠状动脉前降支30 min后复灌30 min,并反复缺血/再灌注3次的方法造成动物急性心肌缺血/再灌注损伤动物模,另取8只不做任何处理作为对照组。采用双抗体夹心法测定兔缺血预处理前、后血清血管内皮因子(Vsculr Endothelil Growthfctor,VEGF)及VEGFmRNA表达;酶联免疫吸附法检验(ELIS)兔血清血清总超氧化物歧化酶(T-SOD)、乳酸脱氢酶(LDH)、肌酸激酶同工酶(CK-MB)及心肌丙二醛(MDA)。籍此评价丹参酮Ⅱ磺酸钠注射液对实验动物心肌缺血预处理后血管内皮损伤的保护作用及其对兔心肌酶谱的影响。结果:4组动物在缺血预处理前各指标比较差异无统计学意义(P>0.05),在使用丹参酮ⅡA磺酸钠注射液缺血预处理后能明显增强缺血预处理A、B 2组兔VEGF及mRNA表达,明显提高缺血预处理A组、B 2组T-SOD、LDH及CK-MB酶的活性,降低2组动物心肌酶谱的MD释放,与缺血/再灌注组比较,差异均有统计学意义(均P<0.05)。缺血预处理A组、B 2组组间比较,差异无统计学意义(均P>0.05)。结论:心肌缺血/再灌注损伤兔在经丹参酮ⅡA磺酸钠注射液缺血预处理后,明显提高VEGF分泌及氧自由基清除酶的活性,加快缺血心肌血管内皮新生,抑制心肌缺血/再灌注损伤时脂质过氧化反应而减轻心肌血管内皮炎性反应,进而改善缺血/再灌注损伤后受损血管内皮功能,对兔急性心肌缺血/再灌注损伤有明确的保护作用。

Objective：To observe the protective effect of Danshen A sodium sulfonate injection on myocardial ischemia and reper- fusion injury in rabbit, investigate the effect of Danshen A sodium sulfonate injection on myocardial enzyme spectrum and explore its potential mechanism to heart function. Methods：Twenty-four rabbits were randomly divided into ischemia/reperfusion group,ische- mic preconditioning processing group A, and ischemic preconditioning group B. Ischemic preconditioning processing group A was injected via ear vein with Danshen A sodium sulfonate injection at 2. 5 mL/kg/d, while ischemic preconditioning treatment group B at 5 mL/kg/d and ischemia/reperfusion group were injected with 1 mL physiological saline. After a week preprocessing,rabbits in all groups were ligated at coronary artery and was re-perfused for 30 minutes ,30 minutes after ligation. The rest 8 rabbits were fed up as control group. Vascular endothelial growth factor （VEGF） and VEGF tuRN expression before and after processing were meas- ured by double antibody sandwich EUS, and serum totM superoxide disnlutase （T-SOD） , lactate dehydrogenase （LDH） , creatine kinase isoenzyme （ CK-MB ） and myocardial malondialdehyde （ MDA ） were measured by enzyme-linked immunosorbent assay （ELIS）. The protective effect of Danshen A sodium sulfonate injection on vascular endothelial injury and its effect on myocardial enzyme spectrum in rabbits after myocardial ischemia preconditioning were evaluated. Results ： Differences of the four groups in is- chemic preconditioning showed no significance （ P 〉 0. 05 ） , VEGF and tuRN expression in both group A and B after preprocessingwere significantly enhanced, and T-SOD, LDH and CK-MB of were obviously improved and the release of MD declined in the two groups with statistically significant difference compared with the ischemia/reperfusion group （ P 〈 0. 05 ）. There was no significant difference between the ischemic preconditioning group A and group B （ P 〉 0. 05 ）. Conclusion ： Danshen A sodium sulfonate injec- tion on myocardial ischemia and reperfusion injury in rabbits may significantly increase VEGF and enhance LDH activity, accelerate myocardial ischemia vascular endothelial growth, inhibit lipid peroxidation inhibition and reduce myocardial vascular endothelial in- flammatory response, thereby improving impaired endothelial function due to ischemia/reperfusion injury. It has clear protective effects on rabbit experimental myocardial ischemia/reperfusion injury.