刺络泻血对高尿酸血症大鼠血尿酸及相关酶活性的影响

Effects of Blood Pricking Therapy on SUA and the Activity of Related Enzyme in Hyperuricemic Rats

目的:观察刺络泻血疗法对高尿酸血症模型大鼠血尿酸及相关酶活性的影响并探讨其机制。方法:将40只清洁级SD大鼠分为正常组、模型组、别嘌呤醇组及刺络泻血组,每组10只,以腺嘌呤、盐酸乙胺丁醇混合液灌胃造模。第4周造模成功后,别嘌呤醇组按照0.03 g/(kg·d)的剂量给大鼠灌胃别嘌呤醇,2次/周;刺络泻血组选取大鼠委中穴及足三里穴穴位附近的血络,出血0.3~0.5 m L,至第8周。第9周后腹主动脉取血,采用磷钨酸法测定血尿酸,采用酶比色法测定黄嘌呤氧化酶(XOD)和腺苷脱氨酶(ADA)的含量。结果:实验结束后,与正常组相比,模型组SUA、ADA、XOD含量均显著增高(P<0.05),差异有统计学意义;与模型组相比,别嘌呤醇组SUA、XOD含量显著降低(P<0.05),刺络泻血组SUA、ADA、XOD含量差异均有统计学意义(P<0.05)。结论:刺络泻血疗法可以有效抑制XOD、ADA活性,降低高尿酸血症大鼠SUA水平。

Objective：To observe the effects of blood pricking therapy on serum uric acid （SUA） and the activity of related en- zyme in hyperuricemic rats, and explore its mechanism. Methods ： A total of 40 male SD rats were randomly divided into four groups：normal group （10 rats） , model group （10 rats） , allopurinol group （10 rats） , and blood pricking group （10 rats）. Rats with hyperuricemic were modeling by adenine and ethambutol hydroehloride intragastrically. Four weeks after successful modeling, rats in allopurinol group were lavaged allopurinol according to the body weight of rats 0. 03 g/L （ kg· d）, 2 times/week ; rats in blood pricking group were pricked for bloodletting 0. 3 - 0. 5 mL on the rats leg collaterals of the Bladder Meridian of the Foot-Taiy- ang and Kidney Meridian of Foot-Shaoyin until the eighth week. At the end of eighth week, the rats were made anesthesia with 10% of chloral hydrate, then 5 mL blood was draw from the abdominal aorta. By phosphoric acid method, the SUA were tested, and with enzyme-eolorimetric method, the activity of the xantbine oxidase （XOD） and adenosine deaminase （ ADA ） were tested. Results ： Compared with the normal group, the levels of SUA, ADA and XOD in the model group were significantly increased （ P 〈 0. 05 ） ; compared with the model group, the levels of SUA and XOD in the allopurinol group were significantly reduced （ P 〈 0. 05 ） ; the changes of SUA, ADA and XOD in the blood pricking group were significant （ P 〈 0. 05 ）. Conclusion： The blood pricking therapy can significantly inhibit the activity of XOD and ADA, reducing the level of SUA in rats with hyperuricemia.