平纤宁肺颗粒剂对肺纤维化大鼠肺组织中转化生长因子β_1和Ⅰ、Ⅲ型胶原表达的影响

Effect of Pingxianningfei Granule on the Expression of TGF-β1,TypeⅠand Type ⅢCollagen in Rats with Pulmonary Fibrosis

目的:观察平纤宁肺颗粒剂对盐酸博莱霉素所致大鼠肺纤维化的保护作用。方法:60只SD大鼠,雄性,按体质量随机分为正常对照组、模型组、平纤宁肺颗粒剂高剂量组、低剂量组,每组15只。盐酸博莱霉素气管内注射诱发肺纤维化,造模后给药,分别在给药7、14、28 d每组处死5只,取肺脏做组织病理学检测,同时免疫组化法检测肺组织内转化生长因子β_1(Transforming Growth Factor-β_1,TGF-β_1)、Ⅰ型胶原、Ⅲ型胶原。结果:模型组大鼠肺组织出现明显的炎细胞浸润,纤维结缔组织增生,平纤宁肺颗粒剂高、低剂量组大鼠在第14、28天的病理变化均较同期模型组减轻。模型组大鼠肺组织TGF-β_1、Ⅰ、Ⅲ型胶原表达在第7、14、28天均明显高于同期正常对照组,与正常对照组比较有统计学意义(P<0.01)。平纤宁肺颗粒剂高、低剂量组TGF-β_1、Ⅰ、Ⅲ型胶表达与同期模型组比较明显降低,有统计学意义(P<0.05,0.01)。结论:平纤宁肺颗粒剂可以有效减轻肺纤维化,抑制肺组织中TGF-β_1、Ⅰ型胶原、Ⅲ型胶原的表达。

Objective：To observe the protection effect of Pingxianningfei Granule in rats with Bleomycin-induced pulmonary fibrosis.Methods：Sixty male SD rats were randomly divided into a control group, a model group（MG）, a high-dose Pingxianningfei Granule group（HG） and a low-dose Pingxianningfei Granule group（LG）, 15 in each.Model rats with Pulmonary Fibrosis were induced by injecting bleomycin into trachea.Respectively, 7, 14, 28 days after successfully medication, the rats were sacrificed, 5 in each group.Taking the lung, and analysis in histopathology.And the expression of transforming growth factor-β1（TGF-β1）, typeⅠand type Ⅲ Collagen in lung tissue was assayed by immunohistochemistry.Results：Infiltration of inflammatory cells were obvious in lung tissue of MG and the fibrous connective tissue proliferated.The pathologic changes of lung in HG and LG at 14, 28 days were milder than those in MG.The expression of TGF-β1, typeⅠand type Ⅲ Collagen in MG at 7, 14, 28 days was much higher than that of the control group, with statistical significance （P〈0.01）.Compared with the MG, the expression of TGF-β1, typeⅠand type Ⅲ Collagen of the lung in HG and LG was decreased, which showed significant difference （P〈0.05, 0.01）.Conclusion：Pingxianningfei Granule can effectively reduce the formation of pulmonary fibrosis and inhibit the expression of TGF-β1, typeⅠand type Ⅲ Collagen in lung tissue.