连翘-4味汤散不同提取物对临床常见致病菌体外抗菌作用研究

Antibacterial Effects of Four Extracts of Lianqiao Siwei Decoction on Common Clinical Bacteria

目的:检测连翘-4味汤散不同溶剂提取物对临床常见致病菌体外抗菌作用。方法:采用琼脂平板稀释法、微孔板TTC法,以连翘-4味汤散不同溶剂(石油醚、氯仿、乙酸乙酯、无水乙醇、75%乙醇、水)提取物为实验药物,选用临床分离鉴定的,耐药现象较普遍的11种常见致病菌株为供试菌,检测实验药物的最小抑菌浓度(MIC)和最小杀菌浓度(MBC)。结果:实验所用6种药物提取物,对金黄色葡萄球菌、表皮葡萄球菌、肠球菌、枯草芽胞杆菌、炭疽芽胞杆菌、大肠埃希菌、肺炎克雷伯菌、鲍曼不动杆菌、铜绿假单胞菌、阴沟杆菌、白假丝酵母菌等11种供试菌种,表现出了较为普遍的抑菌作用和一定的杀菌作用。结论:体外抗菌实验结果显示,实验药物表现出了较高的抗菌效能,连翘-4味汤散做为传统方剂,具有一定的药剂学和药理学研发价值。

Objective：To test the antibacterial effects of four extracts of Lianqiao Siwei decoction on common clinical bacteria. Methods：Using agar plate dilution method and mierowell plate TTC method and taking the extracts of Lianqiao Siwei decoction in different solvent （aether petrolei, chloroform, acetic ether, absolute ethanol, 75 % ethanol and water） as experimental drugs, then selecting 11 common pathogenic strains which are isolated and identified in clinic. To detect the minimum inhibitory concentration （MIC） and the minimum bactericidal concentration （MBC） of them. Results：Six extracts used in the test showed universal inhib- itory effects and certain bactericidal effects against 11 tested strains. They are Staphylococcus aureus, Staphylococcus epidermidis, enterococcus, bacillus subtilis, Bacillus anthracis, Escherichia coli, Klebsiella pneumonia, baumanii, Pseudomonas aeruginosa, Bacillus levans, and Candida albicans. Conclusion ： Different extracts of Lianqiao Siwei decoction have antibacterial effects. As a traditional formula, it worth to be further studied in pharmacodynamics and pharmacology.