袖状胃切除术对高脂饮食诱导肥胖大鼠肠道屏障的影响

Effect of sleeve gastrectomy on intestinal barrier of obesity rats fed with high-fat diet

目的研究袖状胃切除术对高脂饮食诱导肥胖大鼠肠道屏障的影响。方法建立高脂饮食诱导肥胖大鼠模型共30只,随机分为普通饮食组(CD,n=10)、假手术组(SO,n=10)和袖状胃切除组(SG,n=10),术后4周时分别检测24 h尿乳果糖/甘露醇比值(L/M)、门静脉血清内毒素水平和小肠黏膜紧密连接蛋白claudin-1和occludin表达水平。结果术后4周时,SG组大鼠体质量明显低于CD组(P<0.001)和SO组(P<0.001)。SG组大鼠24 h尿L/M值明显低于CD组(P<0.001)和SO组(P<0.01)。SG组门静脉血清内毒素水平明显低于CD组(P<0.01)和SO组(P<0.05)。术后4周时,SG组大鼠小肠黏膜中claudin-1表达水平明显高于CD组(P<0.001)和SO组(P<0.01),occludin表达水平明显高于CD组(P<0.001)和SO组(P<0.001)。结论袖状胃切除术可使高脂饮食诱导的肥胖大鼠体质量下降,降低24 h尿L/M值和门静脉血清内毒素水平,提高小肠黏膜中claudin-1和occludin蛋白的表达水平。

Objective To investigate the effect of sleeve gastrectomy on the intestinal barrier of obesity rats fed with high-fat diet. Methods Thirty obesity rats fed with high-fat diet were randomly divided into three groups including common diet group （ CD, n = 10）, sham operation group （ SO, n = 10） and sleeve gastrectomy group （ SG, n = 10）. The lactulose/mannitol ratios （L/M） in 24-hour urine and endotoxin in portal vein were evaluated four weeks after surgery. The levels of tight junction proteins including claudin- 1 and occludin in intestinal mucosa were ana- lyzed by western blot. Results The body weight of SG group was significantly decreased than those of CD group （ P〈0. 001 ） and SO group （ P〈0. 001 ） four weeks after surgery. The L/M ratio in 24-hour urine of SG group was significantly lower than those of CD group （ P〈0. 001 ） and SO group （ P〈0.01 ）. The endotoxin level in portal vein of SG group was significantly lower than those of CD group （P〈0.01） and SO group （P〈0. 05）. The claudin- 1 level in intestinal mucosa of SG group was significantly higher than those of CD group （P〈0. 001 ） and SO group （P〈0. 01） four weeks after surgery. The occludin level in intestinal mucosa of SG group was significantly higherthan those of CD group （P〈0. 001） and SO group （P〈0. 001 ）. Conclusions Sleeve gastrectomy can reduce body weight, L/M ratio in 24-hour urine and endotoxin level in portal vein of obesity rat fed with high-fat diet and in- crease the levels of claudin- 1 and occludin in intestinal mucosa.