摘要
目的探讨IL-18基因过表达的T细胞对胰腺癌细胞系SW1990的杀伤作用。方法用PCR技术构建含IL-18基因的重组慢病毒,HEK293T细胞包装后感染分离培养的人T淋巴细胞,与胰腺癌细胞SW-1990共培养后,检测培养上清中的LDH含量、ELISA检测IL-2和IFN-γ的含量,以间接评估其对SW-1990细胞体外的杀伤作用。结果成功构建和包装了人IL-18的重组慢病毒,感染人T淋巴细胞后,在与人胰腺癌细胞SW-1990共培养条件下,与空白对照组相比,LDH的释放显著增多(P<0.01);IL-2和IFN-γ的分泌亦显著增多(P<0.01)。结论过表达人IL-18蛋白的T淋巴细胞对胰腺癌细胞SW-1990具有更强的杀伤作用。
Objective To investigate the antitumor effects of IL- 18 gene transfected T cells on pancreatic cancer cell SW- 1990. Methods Construction of IL- 18 contained recombinant lentivirus using PCR method and packaged at HEK293T cell, then transfected the human T cells and evaluated the antitumor effects when cocultured with SW- 1990. Results The IL-18 gene contained recombinant lentivirus was successfully constructed and packaged, and transfected the T cells, the LDH secretion and IL-2 and IFN-γ content all increased significantly (P〈0. 01) when cocuhured with SW-1990 cell. Conclusions T cells transfected with IL-18 possessed more potent antitumor effects to pancreatic cancer cell SW-1990 as compared to the regular T cells.
出处
《基础医学与临床》
CSCD
2017年第9期1251-1256,共6页
Basic and Clinical Medicine
基金
国家自然科学基金青年项目(81201765)
河南省高校重点科研项目(15A320063,17A310022)
新乡医学院研究生科研创新支持计划(YJSCX201509Z)