双岐杆菌减轻盐酸环丙沙星所致的小鼠血清睾酮下降

Bifidobacteria relieve CPFX-induced testosterone reduction in mice

目的探讨双岐杆菌减轻盐酸环丙沙星所致小鼠血清睾酮下降。方法成年雄性昆明小鼠24只,随机分为灌胃0.9%Nacl溶液6 d(Sal6)组、灌胃抗生素6 d(A_6)组、灌胃抗生素6 d后灌胃0.9%Nacl溶液6 d(A_6+Sal_6)组和灌胃抗生素6 d后灌胃双岐杆菌6 d(A_6+P_6)组,每组6只。放免法检测血清睾酮含量;Western blot检测核转录相关因子2(Nrf2)、血红素氧化酶(HO-1)和4羟基壬烯醛(4-HNE)蛋白的表达;real-time PCR检测类固醇激素合成急性调控蛋白(St AR)、胆固醇侧链裂解酶(P450scc)和Nrf2 mRNA的表达;免疫组织化学检测4-HNE蛋白的表达。结果 A_6组血清睾酮水平明显低于Sal6组(P<0.001),A_6+P_6明显高于A_6(P<0.001)和A_6+Sal_6组(P<0.01);睾酮合成酶也表现为同样的变化趋势,A_6组St AR mRNA明显低于Sal6组(P<0.001),A_6+P_6组明显高于A_6组(P<0.01)和A_6+Sal_6组(P<0.01);P450scc mRNA明显低于Sal6组(P<0.001),A_6+P_6组明显高于A_6组(P<0.001)和A_6+Sal_6组(P<0.05);A_6组Nrf2表达明显低于Sal6组(P<0.01),A_6+P_6组明显高于A_6组(P<0.01)和A_6+Sal_6组(P<0.05);A_6组4-HNE的表达明显高于Sal6组(P<0.001),A_6+P_6组明显低于A_6组(P<0.01)和A_6+Sal_6组(P<0.05)。结论双岐杆菌可能是通过调节Nrf2通路,上调抗氧化酶蛋白水平并下调氧化损伤产物,进而减轻盐酸环丙沙星所致小鼠血清睾酮水平下调作用的。

Objective To explore if bacillus bifidus relieve CPFX-induced testosterone reduction in mouse testes. Methods Twenty-four male miees were divided into 4 groups, then administered saline for 6 days （Sal6 group） , CPFX for 6 days （A6 group）, CPFX for 6 days followed by bifidobaeteria treatment for the next 6 days （A6＋P6 group） , CPFX for 6 days and then saline for the next 6 days （ A6 ＋Sal6 group）. We detected serum levels of testosterone by RIA, as well as levels of steroidogenic enzymes mRNA [ cholesterol side-chain cleavage enzyme （P450scc） and steroidogenic acute regulatory protein （STAR） ] and NF-E2-related factor2 （Nff2） mRNA in testes by real-time PCR, Nrf2, heine oxygenase-1 （HO-1）, and 4-hydroxy-2-nonenal （4-HNE） by Western blot and 4-HNE by Immunohistochemistry. Results The A6 group had significantly lower serum testosterone levels compared with the Sal6 group （P〈0. 001）, the A6＋P6 group had significantly higher compared with the A6（P〈0. 001 ） and A6＋Sal6 groups （P〈0. 01 ）. The A6 group had significantly lower StAR mRNA compared with the Sal6 group （P〈 0. 001 ）, the A6＋P6 group had significantly higher level compared with the A6（P〈0. 01） and A6＋Sal6 groups （P〈 0.01 ）. The A6 group had significantly lower P450sec mRNA as compared with the Sal6 group （ P〈0. 001 ） , the A6 ＋P6 group had significantly higher compared with the A6（P〈0. 001） and A6＋Sal6 groups （P〈0.05）. The A6 group had significantly lower Nrf2 compared with the Sal6 group （P〈0. 001 ）, the A6 ＋P6 group had significantly higher compared with the A6 （P〈0. 01 ） and A6＋Sal6 groups （P〈0. 05）.The A6 group higher 4-HNE expression compared with the Sal6 group, the A6＋P6 group had significantly lower compared with the A6（P〈0.01 ） and A6＋Sal6 groups （P〈 0. 05）. Conclusions Bifidobaeteria the reduction of CPFX-indueed testosterone reduction, and these effects may potentially explained by Nrf2 inflammatory signaling pathway.