紫杉醇联合奥沙利铂对非小细胞肺癌模型小鼠的治疗作用

Treatment of paclitaxel combined with oxaliplatin chemotherapy to the non-small cell lung cancer mouse model

目的观察紫杉醇联合奥沙利铂对荷瘤裸鼠非小细胞肺癌治疗效果以及对PDCD5和XIAP表达的影响。方法制备裸鼠肺癌荷瘤模型,将荷瘤小鼠随机分为空白组、0.9%氯化钠溶液组、奥沙利铂组、紫杉醇组和紫杉醇联合奥沙利铂组;q-PCR检测各组PDCD5和XIAP基因表达水平;Western bolt分析各组PDCD5和XIAP蛋白表达情况;对比分析各组肿瘤组织重量。结果紫杉醇联合奥沙利铂组PDCD5 mRNA表达水平最高(P<0.01),XIAP mRNA表达水平最低(P<0.01);紫杉醇联合奥沙利铂组PDCD5蛋白表达最高(P<0.01),XIAP蛋白表达最低(P<0.01);对比各分组肿瘤组织重量,紫杉醇联合奥沙利铂组肿瘤质量最小(P<0.01)。结论紫杉醇联合奥沙利铂化疗能显著增加PDCD5表达和降低XIAP表达,能使已发生的非小细胞肺癌组织质量显著减少。

Objective To investigate the therapeutic effect of paclitaxel plus oxaliplatin chemotherapy to the transplanted non-small cell lung cancer of nude mice and the effect to the apoptosis protein expression of PDCD5 and XIAP with mice model. Methods A tumor-bearing mice were randomly divided into blank group, normal saline group, oxaliplatin group, paclitaxel group, paclitaxel plus oxaliplatin group. The gene expression of PDCD5 and XIAP was assayed by real-time quantitative PCR（q-PCR）. The apoptosis related PDCD5 and XIAP protein were detected by Western blot. Finally, the tumor weight of each group was measured for statistical analysis. Results The mRNA expression of PDCD5 was highest and the gene expression of XIAP was lowest in paclitaxel plus oxaliplatin group（P〈0. 01 ）. The expression of PDCD5 protein was highest and the expression of XIAP protein was lowest in paclitaxel plus oxaliplatin group （ P〈0. 01 ）. Finally, compare the tumor weight of each group, paclitaxel plus oxaliplatin group has the least mass（P〈0. 01 ）.Conclusions Paclitaxel plus oxaliplatin group chemotherapy significantly increases PDCD5 expression and reduce XIAP expression. Meanwhile, paclitaxel plus oxaliplatin chemotherapy can significantly reduce the tumor weight of happened non-small cell lung cancer.