MDV感染B19、B21单倍型SPF鸡免疫器官中miR-146b-5p的变化规律

The Variation of Mir-146B-5P in Immune Organs of B19 and B21 Haplotype SPF Chickens Infect with MDV

目的分析miR-146b-5p在B19(MD易感)与B21(MD抗性)单倍型鸡感染MDV后的表达变化规律,并探讨其可能与MD抗性/易感性相关的分子机制。方法我们在MDV超强毒株Md5攻毒实验和前期高通量测序分析发现,miR-146b-5p表达量有明显差异,为此进一步利用实时荧光定量PCR技术,检测MD感染B19和B21单倍型鸡5dpi、10dpi和21dpi,miR-146b-5p在法氏囊、胸腺和脾脏组织中的表达量变化情况。结果 miR-146b-5p在5dpi、10dpi和21dpi实验组和对照组的表达量明显不同,miR-146b-5p在B21实验组中的表达量要远高于对照组,且差异显著;而miR-146b-5p在B19实验组中的表达量高于对照组但差异不显著。结论 MD感染后,在不同免疫器官中miR-146b-5p的表达量可能存在组织特异性,不同鸡系之间在感染后也存在一定的差异,但具体机制尚需进一步深入研究。

Objective To analyze the miR-146b-5p in B19 （MD susceptible） and B21 （MD resistance） haplotype variation of the expression of chicken infected with MDV and explore the possible after the MD resistance/ susceptibility associated molecular mechanism. Method Through MDV virulent strain Md5 poison attack experiments and previous high-throughput sequencing based differences in the expression of miR-146b-5p on the further use of real-time PCR technology to detect miR-146b-5p in B19 and B21 haplotypes chickens 5dpi, 10dpi and 21 dpi bursa of fabricius thymus and spleen tissues （ experimental group） and the corresponding uninfected group （control group） expression changes. Result MiR-146b-5p was significantly different in experimental group and control group at 5dpi, 10dpi and 21dpi. The expression of miR-146b-5p in B21 group was much higher than that in control group, and the difference was significant. The expression of miR-146b-5p in B19 group was higher than that in control group, but the difference was not significant. Conclusion The expression of miR-146b-5p in different immune organs after MD infection may be tissue-specific, and there are some differences between different chicken lines after infection, but the specific mechanism needs further study.