不育男性年龄与精子核DNA碎片率及精液参数的相关性研究

Study on the relationship between age and sperm DNA fragmentation index and semen parameters

目的:探讨不育男性年龄与精子核DNA碎片率及精液参数的关系。方法:选取于武汉大学人民医院生殖中心就诊的912名不育男性,按照年龄分组,分为三组:年龄20~29岁(A组)、年龄30~39岁(B组)、年龄≥40岁(C组),采用精子核染色体结构检测(SCSA)方法对三组912名男性精子核DNA碎片率(DFI)检测,同时按照WHO第五版标准进行精液常规分析;对年龄与精子核DNA碎片率、精子畸形率、精子密度和前向运动精子活率的关系进行分析。结果:精子浓度、精子畸形率与年龄无明显相关性,前向运动精子百分率与年龄有显著负相关关系,前向运动精子百分率随着年龄升高而显著降低,年龄与精子核DNA碎片率有显著正相关关系,精子核DNA碎片率随着年龄的增加而升高。结论:精子核DNA碎片率及精子前向运动精子百分率会受到年龄的影响,其生育力亦受到年龄的影响。

Objectives： To explore the relationship between age and sperm DNA fragmentation index（ DFI）and semen parameters. Methods： Semen specimens were collected from 912 male patients in our center. All these patients were divided by age into three groups： aged at 20-29（ Group A）,aged at 30-39（ Group B）,aged at ≥40（ Group C）. Sperm chromatin structure assay（ SCSA） was used to detect sperm DNA fragmentation index in three groups. Routine semen analysis also was performed according to the fifth edition of WHO criteria. The relationship between age and DFI,normal sperm morphology rate,sperm motility and sperm concentration was analyzed. Results： Sperm concentration and normal sperm morphology rate had no significant correlation with age. The DFI had a positive correlation with the age,meanwhile progressive motility sperm rate（ PR%） had a negative correlation with age. Conclusion： Age has an impact on sperm DNA fragmentation index and progressive motility sperm rate.