GC基因单核苷酸多态性与宁夏回族绝经后女性血清25-羟维生素D_3水平的研究

Correlation between GC Genetic Polymorphism and 25-hydroxyvitamin D_3 in Ningxia Hui Postmenopousal Women

目的探讨GC基因单核苷酸多态性与宁夏回族绝经后女性血清维生素D水平的关系。方法 2013年10月-2014年11月收集绝经后女性(绝经后组)153例与未绝经女性(绝经前组)56例,采用电化学发光免疫法检测两组研究对象血清25-羟维生素D_3[25-(OH)D)D_3]的水平。直接测序法检测绝经前组和绝经后组GC基因多态性rs222020、rs2298849位点,比较两组之间基因型和等位基因分布。结果绝经后组血清25(OH)D_3水平(15.68±7.385ng·m^(L-1))低于绝经前组(17.43±7.526ng·m^(L-1))(P<0.05)。绝经后组rs222020位点TT基因型和rs2298849位点AA基因型均高于绝经前组,基因型分布差异均有统计学意义(χ~2=8.471、7.374,P=0.0145、0.025),绝经后组rs222020位点T等位基因频率和rs2298849位点A等位基因频率高于绝经前组,等位基因分布频率差异均有统计学意义(χ~2=9.307、8.364,P=0.0023、0.0038)。GC基因222020位点不同基因型间的25(OH)D_3水平差异有统计学意义(F=4.614,P=0.0419),CC基因型人群高于TT基因型(P<0.05)。rs2298849位点不同基因型间的25(OH)D_3水平差异有统计学意义(F=5.172,P=0.0398),GG基因型人群高于AA基因型(P<0.05)。结论宁夏回族绝经后女性存在血清25(OH)D_3不足或缺乏,GC基因rs222020位点的等位基因T和rs2298849位点的等位基因A可能增加机体对维生素D缺乏的易感性。

Objective To explore the correlation of the serum vitamin D levels andGCgene polymorphisms in postmenopausal women of Ningxia Hui ethnicity.Methods153 cases with postmenopausal group＆amp;nbsp;（postmenopausal women）and 56 cases with premenopausal group（premenopausal women）were collected from October,2014 to 2015,November the levels of serum 25（OH）D3 were detected by the electrochemical luminescence immunoassay（ECLI）,to compare the levels of serum 25（OH）D3 between the two groups. Two polymorphisms（rs222020 and rs2298849）ofGCgene were genotyped by direct sequencing,the genotype frequency and allele were compared between the two groups.ResultsThe levels of serum 25（OH）D3 in postmenopausal group（15.68±7.385）ng·mL-1 were significantly lower than those in the premenopausal group（17.43±7.526）ng·mL-1（P〈0.05）. TT genotype of SNPs rs222020 and AA genotype of SNPs rs2298849 in the postmenopausal group were significantly higher than those in the premenopausal group（χ2=8.471/7.374, P=0.0145/ 0.025）. T allele frequency of rs222020 and A allele frequency of rs2298849 in the postmenopausal group were significantly higher than those in the premenopausal group（χ2=9.307/8.364,P=0.0023/0.0038）. The level of 25（OH）D3 in different genotypes in SNPs rs222020 was statistically significant（F=4.614,P=0.0419）, the frequency of CC genotype was higher than TT genotype（P〈0.05）. The levels of 25（OH）D3 in different genotypes in SNPs rs2298849 was statistically significant（F=5.172,P=0.0398）,GGgenotype was higher than AA genotype（P〈0.05）.ConclusionThe Vitamin D3 deficientcy or insufficicney is very common in postmenopousal women of Hui in Ningxia,the allele T of rs222020 and allele A of rs2298849 may increase the body＇s susceptibility to Vitamin D deficiency.