动脉粥样硬化斑块与龈下菌斑中牙周致病菌的关系研究

Correlation of Periodontal Pathogen between Atherosclerotic Plaque and Subgingival Plaque

目的分析动脉粥样硬化斑块与龈下菌斑中牙周致病菌的关系。方法选取2014年8月—2015年10月于新疆医科大学第一附属医院心脏外科行冠状动脉旁路移植术的冠心病并牙周炎患者86例,收集其动脉粥样硬化斑块及龈下菌斑。采用Chelex-100法及聚合酶链反应(PCR)提取动脉粥样硬化斑块及龈下菌斑中福赛斯坦纳菌(Tf)DNA,计算动脉粥样硬化斑块和龈下菌斑中Tf DNA拷贝数,动脉粥样硬化斑块与龈下菌斑中Tf占比的相关性分析采用Pearson相关性分析。结果动脉粥样硬化斑块和龈下菌斑中Tf阳性检出率分别为60.71%、80.21%。动脉粥样硬化斑块和龈下菌斑中Tf DNA测序结果符合Genbank数据库相应细菌标准序列,同源性达99%。动脉粥样硬化斑块中Tf DNA拷贝数为(2.24×10~2±1.52×10~2)拷贝/μl,Tf占比为(0.28±0.13)%;龈下菌斑中Tf DNA拷贝数为(2.06×10~3±1.18×10~4)拷贝/μl,Tf占比为(0.35±0.22)%;动脉粥样硬化斑块和龈下菌斑中Tf占比比较,差异有统计学意义(P<0.05)。Pearson相关性分析结果显示,动脉粥样硬化斑块与龈下菌斑中Tf占比呈正相关(r=0.498,P<0.05)。结论动脉粥样硬化斑块和龈下菌斑中Tf阳性检出率均较高,动脉粥样硬化斑块和龈下菌斑中Tf具有同源性,且动脉粥样硬化斑块与龈下菌斑中Tf相关。

Objective To analyze the correlation of periodontal pathogen between atherosclerotic plaque and subgingival plaque. Methods From August 2014 to October 2015 , a total of 86 coronary heart disease patients complicated with periodontitis were selected in the Department of Cardiac Surgery, the First Affiliated Hospital of Xinjiang Medical University, all of them received coronary artery bypass grafting, atherosclerotic plaque and subgingival plaque were collected. Chelex - 100 method and PCR were used to extract DNA of Tannerella forsythensis （ Tf） in atherosclerotic plaque and subgingival plaque, copies of Tf DNA was calculated in therosclerotic plaque and subgingival plaque, respectively, and Pearson correlation analysis was used to analyze the correlation of Tf ratio beween atherosclerotic plaque and subgingival plaque. Results Positive detection rate of Tf was 60. 71 % and 80. 21 % in atherosclerotic plaque and subgingival plaque, respectively. Sequencing analysis results showed that, Tf DNA sequence in atherosclerotic plaque and subgingival plaque was accord with the standard sequence in Genbank, the homologous rate was 99%. Copies of Tf DNA was （2.24×10^2±1.52×10^2） copies/jjl I [ accounting for （ 0. 28±0. 13） %）in atherosclerotic plaque, copies of Tf DNA was （2.06×10^3±1.18×10^4） copies/jjl I [ accounting for （0.35 ± 0. 22） %〕; the difference of Tf ratio was statistically significantly different between atherosclerotic plaque and subgingival plaque （P 〈0. 05）. Pearson correlation analysis results showed that, Tf ratio in atherosclerotic plaque was positively correlated with that in subgingival plaque （ r = 0. 498, P 〈 0. 05 ） . Conclusion Positive detection rate of Tf is relatively high in atherosclerotic plaque and subgingival plaque, respectively, Tf in atherosclerotic plaque and in subgingival plaque homologous and correlative.