不同品种桑黄抗氧化和抗炎的药理作用比较

Compare of Antioxidant and Anti-inflammatory Activity of Different Phellinus

目的评价桑黄的不同品种火木层孔菌和裂蹄木层孔菌的水提物和醇提物的抗氧化和抗炎作用。方法采用亚铁还原能力(FRAP)和二苯基苦酰肼基(DPPH)自由基清除能力法观察火木层孔菌和裂蹄木层孔菌的的抗氧化作用;采用LPS诱导NO释放法观察火木层孔菌和裂蹄木层孔菌的抗炎作用。结果火木层孔菌和裂蹄木层孔菌水提物的抗氧化能力分别为(374.06±2.29)μmol TEAC·g-1提取物和(355.40±2.18)μmol TEAC·g-1提取物;醇提物的抗氧化能力分别为(408.82±6.91)μmol TEAC·g-1提取物和(414.69±2.58)μmol TEAC·g-1提取物;火木层孔菌和裂蹄木层孔菌均具有明显的清除DPPH自由基的作用,其水提物的IC50分别为7.58 mg·mL^(-1)和8.04 mg·mL^(-1);醇提物的IC50分别为5.32 mg·mL^(-1)和5.15 mg·mL^(-1);火木层孔菌水提物和醇提物作用后,对NO释放量和细胞存活率具有明显的降低作用,其中100、50、25μg·mL^(-1)剂量组与LPS组比较具有显著性差异(P<0.01或P<0.05);裂蹄木层孔菌水提物作用后,对NO释放量和细胞存活率具有明显的降低作用,其中水提物100、50、25、12.5μg·mL^(-1)剂量组与LPS组比较具有显著性差异(P<0.01),醇提物对NO释放量具有明显的降低作用,对细胞存活率具有明显的升高作用,其中25、12.5μg·mL^(-1)剂量组与LPS组比较具有显著性差异(P<0.01)。结论桑黄的不同品种火木层孔菌和裂蹄木层孔菌的水提物和醇提物均具有抗氧化和抗炎作用。

Objective To evaluate the antioxidant and anti-inflammatory activities of water extracts and alcohol extracts of Phellinus igniarius and Phellinus linteus. Methods The antioxidant activity of fungi was determined by ferric reducing antioxidant power （FRAP） and 1-diphenyl-2-picrylhydrazyl （DPPH） radical scavenging activity. The anti-inflammatory activity of the fungi was detected by LPS-induced NO release method. Results In the anti-oxidant activity assay, the Trolox equivalent anti-oxidant capacity （TEAC） of water extracts of Phellinus igniarius and Phellinus linteus were determined to be （374.06±2.29） and （355.40±2.18） μmol TEAC·g -1 extract respectively. TEAC of alcohol extracts were determined to be （408.82±6.91） and （414.69±2.58）μmol TEAC·g-1 extract respectively. DPPH free radical scavenging capability （IC50） of water extracts of Phellinus igniarius and Phellinus linteus were 7.78 and 8.04 mg·mL -1 respectively. DPPH free radical scavenging capability of alcohol extracts of Phellinus igniarius and Phellinus linteus （IC50） were 7.78 and 8.04 mg·mL-1 respectively. Water and alcohol extracts of Phellinus igniarius treatment （100, 50, 25 μg·mL-1） decreased NO formation and cell viability significantly （P 〈0.01 or P〈0.05）. Water extracts of Phellinus linteus treatment （100, 50, 25, 12.5 μg·mL-1） decreased NO formation and cell viability significantly （P〈0.01）. Alcohol extracts of Phellinus linteus treatment （25, 12.5 μg·mL-1） decreased NO formation and increased cell viabil-ity significantly （P〈0.01）. Conclusion The water extracts and alcohol extracts of Phellinus igniarius and Phellinus linteus showed antioxidant and anti-inflammatory activities.