NMBzAz诱导大鼠食管癌动物模型相关的分子机制探讨

Study on NMBzAz Induced Rat Esophageal Cancer Animal Model Related Molecular Mechanism

目的探讨甲基苄基亚硝胺(NMBz Az)诱导大鼠食管癌动物模型相关的分子机制。方法 2015年1月—2016年10月期间,该校病理学教研室选择90只健康清洁级SD大鼠作为研究动物,随机将90只大鼠分为观察组和对照组,各45只。该组大鼠雌雄各半,均为8周龄。观察组大鼠:将甲基苄基亚硝胺(按5 mg/kg)加入2 m L纯净水中,食管内灌注;对照组大鼠:食管灌注2 m L纯净水;两组均灌注1次/周,连续灌注30周。实验后10、20周和30周时间点,两组各宰杀15只大鼠,取食管组织,常规行病理学检查,查看两组食管癌发病情况。同时,提取食管组织总RNA,统计两组不同时间段大鼠survivin m RNA转录水平。结果经病理证实:对照组无大鼠罹患食管癌,观察组实验10周(26.67%)、20周(53.33%)、30周(86.67%)后食管癌发病率均高于对照组,组间差异有统计学意义(P<0.05)。观察组实验10周(0.25±0.09)、20周(0.26±0.11)、30周后(0.24±0.10)食管组织survivin m RNA转录水平均高于对照组[(0.44±0.18)、(0.46±0.16)、(0.47±0.017)],组间差异有统计学意义(P<0.05)。结论 NMBz Az诱导大鼠食管癌动物模型与survivin m RNA转录水平提升存在密切联系,可为食管癌分子靶向治疗提供参考依据。

Objective To study the NMBzAz induced rat esophageal cancer animal model related molecular mechanism.Methods 90 cases of healthy SD rats in the pathological teaching and research room in our school from January 2015 to October 2016 were selected and randomly divided into two groups with 45 cases in each, and half of rats were females and half of rats were males, and all rates were 8 weeks, the rats in the observation group： methylbenzylnitrosamine 5 mg/kg was put into 2ml pure water, esophageal infusion, rats in the control group： esophageal infusion of 2ml pure water, once every two weeks for consecutive 30 weeks, and 15 rats were killed in 10 weeks, 20 weeks and 30 weeks after test and the esophageal tissues were taken and were given the routine pathological examination, and the incidence of esophageal cancer was searched, at the same time, the total RNA of esophageal tissues was taken out, and the survivin mRNA transcriptional level of rats at different time phases was counted. Results The pathology proved that there were no rats with esophageal cancer, and the morbidity of esophageal cancer in 10 weeks（26.67%）, 20 weeks（53.33%）and 30 weeks（86.67%）were higher than those in the control group（P〈0.05）, and the survivin mRNA transcriptional levels in 10 weeks（0.25 ±0.09）, 20weeks（0.26±0.11） and 30 weeks（0.24±0.10） in the observation group were higher than those in the control group [（0.44±0.18）、（0.46±0.16）、（0.47±0.017）], and the differences were statistically significant（P〈0.05）. Conclusion NMBzAz inducedrat esophageal cancer animal model is closely related to the improvement of survivin mRNA transcriptional level,which can provide basis for the targeted treatment of esophageal cancer molecules.