去泛素化酶Usp46基因在系统性红斑狼疮患者血液中的表达及意义

Role of ubiquitin-specific proteases 46 gene in blood of patients with systemic lupus erythematosus

目的研究泛素特异性蛋白酶46(Usp46)基因在系统性红斑狼疮(SLE)患者及健康人外周血中的表达水平,并探讨其在SLE发病中的作用。方法收集73例SLE患者及75例健康人临床资料,采用Real-time PCR法检测外周血中Usp46基因mRNA的表达情况,分析其表达水平与临床指标的关系。结果与健康对照组相比,SLE患者血液中Usp46基因的mRNA表达水平明显升高(Z=-6.98,P<0.01);在SLE患者中Usp46基因的mRNA表达水平与红细胞沉降率(ESR)、IgG、IgM和谷丙转氨酶(ALT)呈正相关(P<0.05)。结论 Usp46基因的mRNA表达水平在SLE患者血液中明显增高,提示Usp46基因可能参与SLE疾病的发生发展过程。

Objective To investigate the role of ubiquitin-specific proteases 46（Usp46）in the blood of systemic lupus erythematosus（SLE）patients and healthy population.Methods Real time polymerase chain reaction analysis was used to determine the Usp46 expression in the blood of 73 patients with SLE and 75 healthy controls.Results The mRNA expression level of Usp46 was significantly increased,about 4.22（1.30,17.02）folds,in samples from SLE patients compared to healthy population（Z =-6.98,P〈0.01）.In addition,there was significantly positive correlation between the expression level of Usp46 and ESR,IgG,IgM and ALT（P〈0.05）.Conclusion The mRNA expression level of Usp46 was significantly higher in SLE patients than in healthy population,which indicates that Usp46 may participate in the development of SLE process.