初治肺结核患者维生素D与其受体基因多态性位点FokI的相关性分析

Association of vitamin D with its receptor genetic polymorphism site Fok I in newly diagnosed pulmonary tuberculosis

目的分析初治肺结核患者血清维生素D(VD)与维生素D受体(VDR)基因多态性位点Fok I的相关性。方法对我院随机选取的180位初治肺结核患者和100位正常对照的血清VD水平,VDR基因多态性位点Fok I基因型进行检测,比较和分析2组血清VD水平与VDR基因Fok I多态性差异以及相关性。结果初治肺结核患者血清VD水平明显低于正常对照组(P<0.0001);初治肺结核组VD营养缺乏率(70.5%)明显高于正常对照组(47.0%),VDR基因多态性位点Fok I的三种基因型(纯合子FF,纯合子ff和杂合子Ff)在两组内的分布差异具有统计学意义(P=0.033),初治肺结核组纯合子ff及等位基因f比率明显高于正常对照组(P<0.05);初治肺结核患者基因型ff组VD水平明显低于基因型FF组和基因型Ff组(P<0.05)。结论初治肺结核患者血清VD水平明显下降,VDR基因多态性位点Fok I基因型ff为肺结核易感基因。

Objective To analyze the correlation between vitamin D( VD) and vitamin D receptor( VDR)genetic polymorphism site Fok I in newly diagnosed pulmonary tuberculosis patients. Methods 180 newly diagnosed pulmonary tuberculosis patients( PTB) and 100 healthy control were randomly selected. Their serum VD levels and VDR genetic polymorphism site Fok I were detected and compared. Results Serum VD level was significantly lower in the PTB group than in the control group( P < 0. 0001). The deficiency of VD was significantly higher in the PTB group( 70. 5%) than in the control group( 47. 0%). The difference of the three genotypes( homozygous FF,homozygous ff and heterozygous Ff) of VDR polymorphism site Fok I in the two groups was statistically significant( P =0. 033). The rate of homozygous ff and allele f in the PTB group was significantly higher than that in the control group( P < 0. 05). The level of VD in the ff group was significantly lower than in the FF group and the Ff group in PTB( P< 0. 05). Conclusion The level of serum VD decreases significantly in newly diagnosed PTB. VDR genetic polymorphism site Fok I genotype ff is the susceptibility gene of PTB.