布加综合征患者腔房转流术后血清MAPK相关蛋白的表达及意义

Expressions of serum MAPK-related proteins in patients with Budd-Chiari syndrome after artificial vascular bypass operation and their clinical significance

目的观察布加综合征(BCS)患者腔房转流术后血清丝裂原活化蛋白激酶(MAPK)相关蛋白的表达。方法选取腔房转流术后BCS患者(观察组)和健康志愿者(对照组),各40例。采用Western blot检测术前和术后3 d及1个月血清MAPK相关蛋白表达。结果 (1)观察组术前和术后各时间点血清胞外信号调节激酶1/2(ERK1/2)、p38、c-Jun氨基末端激酶及胞外信号调节激酶5(ERK5)的表达水平与对照组比较,差异无统计学意义(P>0.05),且观察组术前表达水平与术后3 d及术后1个月比较,差异无统计学意义(P>0.05);(2)观察组术前和术后各时间P-ERK1/2、P-p38及磷酸化c-Jun氨基末端激酶(P-JNK)表达水平均高于对照组,P-ERK5低于对照组;(3)观察组术后3 d的P-ERK1/2、P-p38及P-JNK表达水平较术前上升,而P-ERK5较术前下降。结论腔房转流术可上调BCS患者血清P-ERK1/2、P-p38及P-JNK的表达水平,下调血清P-ERK5的表达水平,而对各总蛋白的表达无调控作用。

Objective To observe the expressions of related proteins of serum mitogen activated protein kinase （MAPK） in patients with Budd-Chiari syndrome （BCS） after artificial vascular bypass operation and discuss their clinical significance. Methods In this study, 40 BCS patients （observation group） and 40 healthy volunteers （control group） were collected. The raleted proteins of serum MAPK were tested by Western blot before operation, 3 days and 1 month after operation, and compared between the two groups. Results The expression levels of serum extracellular signal-regulated kinases 1/2 （ERK1/2）, P38, JNK and ERK5 in the observation group before operation, 3 days and 1 month after operation were not significantly different from those in the control group （P 〉0.05）. In the observation group the expression levels of ERK1/2, P38, JNK and ERK5 before operation were not significantly different from those 3 days or 1 month after operation. Compared to the control group, the expression levels of serum P-ERK1/2, P-P38 and P-JNK were higher, while P-ERK5 was lower in the observation group at each time point （P 〈0.05）. In the observation group, the expression levels of serum P-ERK1/2, P-P38 and P-JNK rised, but the P-ERK5 level reduced 3 days after operation compared to the preoperative levels. Conclusions Artificial vascular bypass operation can increase the expression levels of serum P-ERK1/2, P-P38 and P-JNK, but reduce serum P-ERK5 expression.However, it has no obvious regulatory effect on the total protein expressions.