不同光环境下棉花叶片色素含量和荧光参数与光谱参数的相关性

Correlation between Pigment Content,Fluorescence Parameters and Spectral Parameters of Cotton Leaves in Different Light Environments

【目的】研究不同光环境下,棉花不同发育阶段叶片色素含量、荧光参数和光谱参数之间的关系,通过光谱参数快速无损估测叶片光合生理指标。【方法】棉花品种为新陆早45号,对棉花不同发育阶段叶片进行遮荫处理,分别测定其色素含量、荧光参数、光谱反射率,并进行相关性分析。【结果】与对照相比,遮荫后棉花不同发育阶段叶片550 nm处反射率显著升高,680 nm处反射率差异不显著,780~1 100 nm间反射率显著降低。棉花叶片色素含量和荧光参数与光谱参数均具有显著的相关性。【结论】RRed/RGreen可以估测不同光环境下棉花不同发育阶段叶片类胡萝卜素含量的变化。PSSRa、PSSRb和m SR705均可以估测不同光环境下棉花不同发育阶段叶片与光化学过程相关的参数,如Y(II)、q P和q L的变化,而PRI可以估测与非光化学过程相关的参数,如NPQ、q N和Y(NPQ)的变化。

[Objective] The purpose of this study is to estimate leaf photosynthetic physiological indexes with fast speed and no damage by studying spectral parameters and the relationship between chlorophyll con-tent, chlorophyll fluorescence parameters and spectral parameters in different growth stages of cotton in differ-ent light environments.[Method] The cotton （Xinluzao No. 45） leaves at different developmental stages were treated with shading, the pigment content, fluorescence parameters and spectral reflectance were measured and the correlation analysis was carried out.[Result] Compared with the CK, the reflectance was increased at 550 nm,not significant at 680 nm and decreased at 780 -1,100 nm in different growth stages of cotton leaves in different light environments. There were significant correlations between pigment content, fluorescence param-eters and spectral parameters of cotton leaves.[Conclusion] RRed /RGreen can be used to estimate the changes of carotenoid content in different growth stages of cotton leaves in different light environments. PSSRa, PSSRb and mSR705 can be used to estimate the changes of the parameters associated with photochemical processes at different growth stages of cotton leaves in different light environments, for example Y （ II） , q P and q L , while PRI can be used to estimate the changes of the parameters associated with non photochemical processes, for example NPQ , q N and Y （ NPQ）.