四株解磷菌分泌有机酸与溶解磷酸三钙能力的研究

Study on Organic Acid Secreted from 4 Strains Phosphorus-solubilizing Bacteria and Calcium Phosphate Dissolving Ability

【目的】研究解磷细菌溶解难溶性磷酸盐及溶磷过程中有机酸的作用。【方法】对P7、P13、P15、P18四株解磷菌进行培养,并对其进行动态观测,用高效液相色谱法测定其有机酸种类及含量,研究其解磷量与有机酸之间的关系。【结果】4株解磷菌溶解磷酸三钙能力有显著差异,解磷能力大小依次是P15>P13>P18>P7。4株解磷菌产生有机酸的含量差异很大,并且同一株解磷菌在培养过程中产生的有机酸种类也会不同。经分析发现有机酸对速效磷的直接影响由强至弱排序依次是:丙酮酸、酒石酸、乳酸、乙酸、草酸。【结论】解磷能力最强的是P15菌株,丙酮酸对溶解磷酸三钙的直接影响系数最大。

【 Objective】 To study organic acid secreted from phosphorus - solubilizing bacteria and calci-um phosphate dissolving ability.【 Method】 The P7, P13 , P15 and P18 phosphate - solubilizing bacteria were sent to cultivate for dynamic observation. The organic acid types and content were determined by high perform-ance liquid chromatography （ HPLC ） method for studying the relationship between phosphate - solubilizing and organic acids.【 Result】 The research showed that dissolving calcium phosphate capacity of the 4 strains of bacteria had significant difference, the order of which was： PI5 〉 PI3 〉 P18 〉 P7 and the content differ-ence of four kinds of bacteria producing organic acids was also great, and organic acids produced by the same bacteria at different times were different, too. Through the path analysis, it was found that the direct effects of organic acids on available phosphorus in descending order were ： pyruvic acid 〉 tartaric acid 〉 lactic acid 〉 acetic acid 〉 oxalic acid.【 Conclusion】 The strongest ability to solve phosphorus bacteria is the P15 strain and the direct influence coefficient of pyruvic acid on the dissolution of calcium phosphate is the largest.