耐辐射短梗霉发酵黑色素动力学模型

Fermentation Kinetic Model of the Melanin Produced from a Radiation-resistant Aureobasidium pullulans

【目的】研究耐辐射短梗霉MF1黑色素分批发酵动力学。【方法】描述短梗霉分批发酵黑色素过程中细胞生长、产物积累、糖消耗的变化规律。基于Logistic方程和Luedeking-Piret方程分别建立黑色素发酵过程菌体生长、黑色素产生及葡萄糖消耗随时间变化的数学模型,对实验值与模型进行了验证比较。【结果】动力学模型计算值结果与实验值拟合良好,较好地反映了短梗霉黑色素分批发酵过程的动力学特征。计算获得菌体最大黑色素生产能力为1.523×10-4g/g葡萄糖·g干菌体·h。【结论】该动力学模型为天然菌株黑色素的中试放大,大规模生产及应用奠定了基础。

【 Objective】 In this paper, the melanin produced by the batch fermentation of a radiation - re-sistant MF1 and fermentation kinetics is studied.【 Method】 Cell growth, melanin pro-duction and glucose consumption throughout the melanin fermentation were investigated. The kinetic model was built to describe the cell growth, melanin production and glucose consumption based on Logistic equation and Luedeking - Piret equation. And the predictive values of models were compared with the experimental da-ta. 【 Result】 Results showed that kinetic properties in the production process of melanin by batch fermenta-tion. The calculated result of model fit well with the experimental data. And calculated maximum melanin pro-duction of cell was 1. 523 × 10^-4 g/ggiucose · gdry cell· 【Conclusion】 The kinetic model laid foundation forthe pilot scale, large - scale production of water - soluble natural melanin in the future.