茯苓皮水提物对四氯化碳诱导大鼠肝纤维化的改善作用

Improvement Effect of Poria cocos Peels Water Extract on Liver Fibrosis in Rats Induced by Carbon Tetrachloride

目的:研究茯苓皮水提物(PWE)对四氯化碳(CCl4)诱导大鼠肝纤维化的改善作用。方法:将84只大鼠随机分为空白对照组、溶剂对照组、模型对照组、阳性对照组(复方鳖甲软肝片,0.75 g/kg)和PWE低、中、高剂量组(0.9、1.8、3.6 g/kg,以生药计),每组12只。除空白对照组与溶剂对照组(ip植物油)外,其余各组大鼠均ip CCl_4-植物油溶液复制肝纤维化模型。造模成功后,各给药组大鼠ig相应药物,其余3组大鼠ig等体积生理盐水,每天1次,连续4周。给药结束后,采用酶联免疫吸附法检测大鼠血清中天冬氨酸转氨酶(AST)、丙氨酸转氨酶(ALT)、层黏连蛋白(LN)、透明质酸(HA)、羟脯氨酸(Hyp)和肝组织中还原性谷胱甘肽(GSH)、超氧化物歧化酶(SOD)、丙二醛(MDA)的含量,苏木精-伊红染色和Masson染色观察大鼠肝组织病理变化。结果:与空白对照组比较,溶剂对照组大鼠各指标均无明显变化(P>0.05);模型对照组大鼠血清中AST、ALT、LN、HA、Hyp含量和肝组织中MDA含量显著升高(P<0.05),肝组织中GSH、SOD含量显著降低(P<0.05),且肝组织发生明显纤维化病变。与模型对照组比较,PWE中、高剂量组大鼠血清中AST、ALT、LN、HA、Hyp含量和肝组织中MDA含量显著降低(P<0.05),肝组织中GSH、SOD含量显著升高(P<0.05),肝组织纤维化程度明显减轻。结论:PWE对CCl_4诱导的大鼠肝纤维化具有良好的改善作用,其机制可能与抑制机体脂质过氧化有关。

OBJECTIVE：To study the improvement effect of Poria cocos peels water extract（PWE）on liver fibrosis in rats induced by carbon tetrachloride（CCl_4）. METHODS：84 rats were randomly divided into blank control group,solvent control group,model control group, positive control group（Compound biejia ruangan tablet, 0.75 g/kg）, PWE low-dose, medium-dose,high-dose groups（0.9,1.8,3.6 g/kg,calculated by crude drugs）,12 in each group. Except for blank control group and solvent control group（ip vegetable oil）,other groups received CCl_4-vegetable oil solution to reduce liver fibrosis model,ip. After modeling,each administration group received related medicines,ig,other 3 groups received equal volume of normal saline,once a day,for 4 weeks. After administration,enzyme-linked immunosorbent assay was used to detect the aspartate aminotransferase（AST）,alanine aminotransferase（ALT）,laminin（LN）,hyaluronic acid（HA）,hydroxyproline（Hyp）contents in serum and reduced glutathione（GSH）,superoxide dismutase（SOD）,malondialdehyde（MDA）contents in liver tissue of rats;HE staining and Masson staining were adopted to observe the pathological changes of liver tissue. RESULTS：Compared with blank control group,indexes of rats in solvent control group had no obvious changes（P〈0.05）. AST,ALT,LN,HA,Hyp contents in serum and MDA content in liver tissue in model control group were significantly increased（P〈0.05）;GSH,SOD contents in liver tissue were significantly reduced（P〈0.05）;and liver tissue showed obvious fibrosis lesions. Compared with model control group,AST,ALT,LN,HA,Hyp contents in serum and MDA content in liver tissue in PWE medium-dose,high-dose groups were significantly reduced（P〈0.05）;GSH,SOD contents in liver tissue were significantly increased（P〈0.05）;fibrosis degree of liver tissue was obviously relieved. CONCLUSIONS：PWE shows good improvement effect on liver fibrosis of rats induced by CCl_4,which may be related to inhibiting the lipid peroxidation.