乙醇诱导对鼠李糖乳杆菌HCCB 20591代谢乙醛的影响

Effect of Ethanol-induction on the Metabolism of Acetaldehyde in Lactobacillus rhamnosus HCCB 20591

乙醛是酒类饮品中的风味物质,但乙醛过高不仅会影响酒类饮品的风味,还会对人体产生潜在的危害。从可食用的益生菌中筛选可以代谢乙醛的菌株,并探究活性菌株的乙醛代谢能力跟乙醇诱导的关系。通过静息细胞法筛选获得的鼠李糖乳杆菌HCCB 20591具有较强代谢乙醛的能力,且其代谢能力受乙醇诱导。当乙醇诱导浓度为3%,诱导时间为12 h时,乙醛转化率达到41.13%。GC-MS分析其代谢产物除了乙酸外,还生成了乙醇。采用Real time RT-PCR方法分析相关基因的转录水平,发现乙醛代谢是乙醇脱氢酶、乙醛脱氢酶和醛氧化还原酶综合催化作用的结果。

Acetaldehyde is a flavor substance in alcoholic beverages. Excessive acetaldehyde not only affects the flavor of alcoholicbeverages,but also causes health damages to human body. This study is to screen edible probiotic strains with the ability to metabolizeacetaldehyde,and explore the relationship between acetaldehyde metabolism and ethanol-induction. Strain HCCB 20591 of Lactobacillus rhamnosus with strong acetaldehyde metabolism ability was obtained via a resting cell system,and its metabolic ability was induced by ethanol.When the ethanol concentration was 3% and the induction time was 12 h,the acetaldehyde conversion ratio by HCCB 20591 was 41.13%.GC-MS analysis of the acetaldehyde metabolites showed that in addition to acetic acid,ethanol was also generated by HCCB 20591. RT-PCRmethod was used to analyze the transcription level of the related genes. It was found that acetaldehyde metabolism was the combined catalyticresult of alcohol dehydrogenase,aldehyde dehydrogenase and aldehyde oxidoreductase.